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Inoculation of clinical sample in embryonated egg and in laboratory animals

Inoculation of clinical sample in embryonated egg and in laboratory animals. By Dr. Hesnaa Saeed Al-Mossawi. Inoculation of clinical sample in embryonated egg. Routs: Chorioallantoic membrane CAM (HSV, Pox) Amniotic cavity (influenza, mumps) Allantoic cavity Yolk sac. Procedure.

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Inoculation of clinical sample in embryonated egg and in laboratory animals

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  1. Inoculation of clinical sample in embryonatedegg and in laboratory animals By Dr. Hesnaa Saeed Al-Mossawi

  2. Inoculation of clinical sample in embryonated egg • Routs: • Chorioallantoicmembrane CAM (HSV, Pox) • Amniotic cavity (influenza, mumps) • Allantoic cavity • Yolk sac

  3. Procedure 1- Candle the egg Three days aged embryo

  4. Five days aged embryo Died embryo

  5. 2- Drill a slit in the egg shell

  6. 3- Using sterile syringe inoculate 0.1ml of specimen

  7. 4- Seal the opening and rotate the egg

  8. 5- Incubate the egg at 37C Incubator 37ºC

  9. 6- Harvest the egg looking for pocks on CAM (chorioallantoic membrane)

  10. Recognition • Death of the embryo • Pocks (differentiate between HSV and Pox ,VS, HSV-1 and HSV-2)

  11. Pocks on CAM (chorioallantoic membrane): chick embryo infected with HSV-1 show pocks on CAM.

  12. Inoculation of clinical sample in Lab animals • Include: • Mice , rabbit , monkey • Used for isolation of • Arbovs, HSV, Coxsacki A and B

  13. Procedure 1- Inoculate newborn mice within 24-48 hours of birth using long-needle syringe * Intracerebral

  14. * Intraperitoneal

  15. 2- Check inoculated mouse for signs of illness, paralysis, or death. • Flaccid paralysis • Spastic paralysis Recognition: Death, paralysis, hemorrhage Identification: Immunoassay

  16. Uses of Animal Systems in Virology: 1.When the virus cannot be propagated in vitro 2.To study the pathogenesis of viral infections 3.To study vaccine safety But Animal Systems are of little use now adaysfor the following reasons: 1. Expensive and time consuming 2. Whole animal is a complex system 3. Results are not always reproducible due to host variation 4. Virus isolation in animals is inferior to the molecular techniques like PCR.

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