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J37019 + I13033

Ligation Chart. FOR. Step 1. Step 2. Step 3. Step 4. J3701 (Alternate Method). C0261 + I13504. F2620+(C0261+I13504) MAKES PART J37015!. J37016 Predator cell transfer function test (poly-cistronic). J37019 + I13504 MAKES PART J37016 !!. B0034+C0062. J37019 Intermediate Part.

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J37019 + I13033

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  1. Ligation Chart FOR Step 1 Step 2 Step 3 Step 4 J3701 (Alternate Method) C0261 + I13504 F2620+(C0261+I13504) MAKES PART J37015! J37016 Predator cell transfer function test (poly-cistronic) J37019 + I13504 MAKES PART J37016 !! B0034+C0062 J37019 Intermediate Part R0062 + (B0034+C0062) Add Aiia part for final construct (for monocistronic mRNA) J37020 Predator Transfer Function (mono-cist) Wait for part I13033 to grow! I13033 + I13504 J37019 + _______ MAKES PART J37020 !! J37020 (Alternate Method) J37019 + I13033 Add I13504 to get part J37020(to test previous ligation worked ) Add Aiia part for final construct

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