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The ProteomeWorks System ~ New Mass Spectrometry Systems ~

The ProteomeWorks System is the global alliance of Micromass and Bio-Rad Laboratories dedicated to furthering proteomics research. The ProteomeWorks System ~ New Mass Spectrometry Systems ~.

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The ProteomeWorks System ~ New Mass Spectrometry Systems ~

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  1. The ProteomeWorks System is the global alliance of Micromass and Bio-Rad Laboratories dedicated to furthering proteomics research.

  2. The ProteomeWorks System~ New Mass Spectrometry Systems ~ At Pittcon 2001 Micromass introduced a range of e-MSTM enabled MALDI-TOF MS / MS-MS & ESI LC-MS-MS systems and Mass-InformaticsTM Software to leverage The ProteomeWorksTM System • M@LDI HT TM • Q-Tof UltimaTM • Q-Tof microTM

  3. M@LDI HT TM [new instrument] Micromass’ newnetworked MALDITOF-MS & sample management system “2D-gel-MS analyser for proteomics factories”

  4. M@LDI HT TM - Networked MALDI-TOF MS LINEAR Intact Proteins REFLECTRON Peptide Mass Fingerprinting

  5. VERTICAL FLIGHT TUBE AND REFLECTRON HOUSING VERTICAL AXIS MOTOR HORIZONTAL AXIS MOTOR X - Y ROBOTIC ARM ELECTRO MAGNETIC GRIPPER IN COLUMN OUT COLUMN TARGET PLATE CASSETTE 2 TARGET PLATE CASSETTE 1 BAR CODE SCANNER VACUUM LOCK LID M@LDI HT TM - Sample Management System80 x 96/384-Sample Targets (bar coded)

  6. M@LDI HT TM - Networked MALDI-TOF MS • Linear and reflectron ion optics options • 80 x 96/384-Sample target management system • Bar coded sample target plates • Automated Laser Positioning System (ALPS) for sample target alignment and sample indexing • Intelligent acquisition / calibration algorithm • Web-enabled Client-Server Mass-Informatics for interrogation of non-redundant protein dB …converts samples into knowledge not ‘just’ spectra

  7. ProteinLynxTM Global SERVER web-enabled Mass-informatics

  8. ProteinLynxTM Global SERVER export to PDQuest Annotation Tools

  9. C L I C K PDQuest Annotation Tools

  10. Technique Data Type Dynamic Specificity Speed Data Interpretation Constraints Range MS MALDI Low Low High Known Protein dB + MH MALDI PSD Low Low Medium Known Protein dB + MH MS-MS MALDI Low Medium High Known Protein dB + MH Known nucleotide dB Fragments ESI + HPLC High High Medium Known Protein dB ++ MH & 2 Known nucleotide dB Fragments *ESI + HPLC (de novo) Amino Acid High High Medium Homologues (BLAST) Sequence Time (PCR) MS & MS-MS Techniques for Peptide / Protein Analysis

  11. Q-Tof UltimaTMfamily[new instruments] Micromass’ SuperPremium family of MALDI MS-MS and ESI MS-MS systems for PROTEOMICS and structural analysis DDATM

  12. Q-Tof Ultima…the SuperPremium family of MS-MS • Q-Tof Ultima MALDI • The high throughput MALDI MS-MS • Q-Tof Ultima API • The SuperPremium API LC-MS-MS • Q-Tof Ultima GLOBAL • The flexible research platform for API LC-MS-MS / MALDI MS-MS DDATM

  13. Q-Tof UltimaTM MALDI [new instrument] • Dedicated MALDI MS-MS system • V & W-OPTICS options • ProteinLynx Global SERVER • e-MS enabled

  14. Q-Tof Ultima MALDI with VOPTICS 10,000 FWHM

  15. Q-Tof Ultima MALDI with WOPTICS 20,000 FWHM

  16. MALDI MS-MS is superior to PSD ADH 1 pm/µl PSD ADH 1 pm/µl Q-Tof Ultima MALDI MS-MS

  17. ProteinLynxTM Global SERVER ...Matching MS-MS spectra to dB of known protein or nucleotide sequence.

  18. Q-Tof UltimaTM API [new instrument] • Dedicated API (ESI) LC-MS-MS system • V & W-OPTICS options • DDA - Precursor Ion Discovery (patent applied for) • “Neutral Loss” • “Precursor Ion” • High resolution • Exact mass • Compatible with HPLC • ProteinLynx Global SERVER • e-MS enabled

  19. Q-Tof Ultima API with WOPTICS 20,000 FWHM

  20. Q-Tof UltimaTM API ...comparison of V & W-OPTICS Resolution

  21. The superior peptide sequence coverage provided by ESI MS-MS data enables de novo sequencing

  22. Sequencing results from MS-MS of m/z 841.5

  23. Q-Tof UltimaTMGLOBAL[new instrument] • Flexible API & MALDI MS-MS research platform • API / MALDI changeover without venting • V & W-OPTICS options • LC-DDA-MS • Precursor ion discovery • “Neutral Loss” • “Precursor Ion” • Exact mass • ProteinLynx Global SERVER • e-MS enabled

  24. Q-Tof Ultima GLOBAL

  25. Q-Tof microTM[new instrument] Micromass’ finest API Q-Tof technology for structural analysis & protein characterisation… • SMALLER • SMARTER • WIRED DDATM

  26. Q-Tof microTM…SMALLER • ZSPRAYTMnanoflow ESI inlet technology • 5000 FWHM resolution • 5ppm RMS exact mass measurement • Dynamic External Calibration (DXCTM) • 20000 m/z range

  27. Q-Tof microTM …SMARTER …WIRED • Data Directed Analysis (DDA) • Automates analysis of protein digests by LC-DDA-MS • Precursor Ion Discovery (patent applied for) • “Neutral Loss” • “Precursor Ion” • High resolution • Exact mass • Compatible with HPLC • Maximising data quality

  28. Data Directed Analysis DDATM Exclusion of 1+ background ions

  29. The New e-MSTM Enabled Mass Analysers of The ProteomeWorksTM System... msinnovation.com

  30. The ProteomeWorks System is the global alliance of Micromass and Bio-Rad Laboratories dedicated to furthering proteomics research.

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