1 / 20

Effects of Indigofera tinctoria extracts on macrophage activation and lymphocyte proliferation

This study evaluates the in vitro effects of ethanolic and aqueous extracts of Indigofera tinctoria on macrophage activation and lymphocyte proliferation. The results show enhanced macrophage functions and lymphocyte proliferation, indicating the potential immunomodulatory effects of this plant.

laverneh
Download Presentation

Effects of Indigofera tinctoria extracts on macrophage activation and lymphocyte proliferation

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Effect of Indigoferatinctoriaethanolic and aqueous extracts on in vitro macrophage activation and lymphocyte proliferation • M.Boothapandi • Research Scholar • Department of Zoology • University of Madras • Chennai • Prof. R. Ramanibai • Research Supervisor & Guide • Department of Zoology • University of Madras • Chennai

  2. INTRODUCTION • Immunomodulators are drugs which either suppress or stimulate the immune system – (Immunosuppressants and Immunostimulants) • Immunostimulants • Chemical agents - levomisole, Cimetidine, Isoprinosine(ISO) • Microbiological agents- BCG ( bacille Calmette-Guerin,) • Indication- Tumor, Immunodeficiency, Infectional diseases • Immunosuppressant • Chemical agents- Cyclophosphamide, Azathioprine • Hormone- steroids • Microbiological agents- Cyclosporine A(CsA) • Indication- Organ transplantation, Hypersensitivity diseases, Autoimmune diseases, Infectious diseases

  3. Medicinal uses of I.tinctoria Antiproliferative activity in human lung cancer (Kameswaran and Ramanibai, 2008) Root and leaves-To treat epilepsy and hydrophobia (Tyagiet al., 2010) Indigotin- Hepatoprotective activity (Singh et al., 2001) Indirubin- Anticancer drug (Han, 1994)

  4. OBJECTIVES To evaluate the effect of ethanolic and aqueous extract of I.tinctoriaon in vitro Macrophage functions. To estimate theeffect of ethanolic and aqueous extract of I.tinctoriaon in vitro lymphocyte proliferation

  5. Systematic position of the plant Kingdom :Plantae Division : Magnoliophyta Class : Magnoliopsida Order : Fabales Family : Fabaceae Genus : Indigofera Species : tinctorialinn.

  6. MATERIALS AND METHODS Extract preparation – Aqueous extract and ethanolic extract were prepared in respective solvents Macrophage isolation from peritoneal fluid and lymphocytes isolated from Peripheral blood (Cheng et al., 2008) of wistar albino rat. Nitric Oxide (NO) production (Green et al., 1982) Arginase activity (Munder et al., 1998) Pinocytic activity (Plytycz et al., 1992) Lysosomal activity (Suzuki et al., 1990) Phagocytic assay on nitroblue tetrazolium (NBT) reduction Test (Rainard, 1986) Lymphocytes proliferation (Mossmann, 1983)

  7. RESULTS

  8. A B Field Emission- Scaning Electron microscope (FE-SEM) image of isolated (A) peripheral blood lyphocytes and (B) peritoneal macrophage (PEM) from wistar albino rat

  9. Effect of I.tinctoria extracts on PEM Nitric Oxide (NO) Production and Arginase activity

  10. Effect of I.tinctoria extracts on PEM Pinocytic Activity

  11. Effect of I.tinctoria extracts on PEM Lysosomal Activity

  12. Effect of I.tinctoria extracts on PEM Phagocytosis

  13. Effect of I.tinctoria extracts on invitro proliferation

  14. Effect of I.tinctoriaextracts on invitroproinflammatory cytokine production

  15. SUMMARY • In innate immune system both aqueous ethanolic and aqueous extracts were enhanced the Macrophage NO production and simultaneously decrease the activity of Arginase. • The other predominant activity of PEM like Pinocytic and lysosomal activity also substantially enhanced by both ethanolic and aqueous extracts • The principal role of Macrophage phagocytosis also notably enhanced. • On the other hand the it enhance the adaptive immune system through the proliferation of lymphocytes.

  16. OUTCOME • The present findings revealed that both the ethanolic and aqueous extracts of I. tinctoria did not cause direct toxic effects on isolated immune cells and suggested that they could potentially enhance innate as well as adaptive immune responses. • These strong immunomodulatory activities of I. tinctoria noted here in vitro help to explain some of the medicinal benefits attributed to the plant in traditional medicine and could be used to provide a future basis for the development of this plant as an immuno-regulating substance.

  17. Future Plan of the work.... • To find out the active compounds responsible for the immunomodulatory potential of I.tinctoria and find out the immunomodulatory effect through invivo studies on Wistar albino rat model.

  18. ACKNOWLEDGEMENT We acknowledge to UGC- UPE – PHASE II New Delhi (No. 2013/PFEP/C3/280) for financial support.

  19. THANK YOU

More Related