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DNA????????,????????????????????????????1924?Feulgen?????DNA-Feulgen??????,????????DNA?????????????? ?????? 1 ??Feulgen???????? 2 ??????????????????????? 3 ?????????????? . ??????. DNA???(1mol/LHCL)??,?????????????????,???????????????,????????Schiff??(?????????)??,?????????,?
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1. ??? DNA?Feulgen?????????????
2. DNA????????,????????????????????????????1924?Feulgen?????DNA-Feulgen??????,????????DNA??????????????
??????
1 ??Feulgen????????
2 ???????????????????????
3 ??????????????
3. ??????
DNA???(1mol/LHCL)??,?????????????????,???????????????,????????Schiff??(?????????)??,?????????,??????DNA?????????
4. ????
1. Carnoy??? :3?95%????1?????
2. 1mol/LHCL :????86.2ml???(??1.18)???93.8ml?????
3. Schiff??:?1g?????200ml????????,5??????????55~50?,????????????,??1N HCl 20ml,?????25?,??1g??????,?????????????,??????????(4???),18~24?????,?????????????????,???Schiff????????????????,???1g???,???????,?????????,???????????????,????,?5?????????????
5. 4. ?????? (???):?200ml????,??10ml 1N HCl ?10ml 10%?????????(?1.0g??)?????????? ????SO2????????
5. 5%????
6. 0.5%??????(95%?????)
6. ???????????
1. ??
Carnoy????????
??????????
??????????
2. ????
???????????????????????? ??????????????????????????????????????????
7. ?????????
(?)???? ??????,????????????????????????1cm?,???????Carnoy??????????
(?)?? ???,???????????????,???????????,?????3?,?????3?,?1mol/L HCl???,??,????60??1mol/L HCl 3ml,?????????60±0.5????8-15??(?????,???????8?????30??)??????1mol/L HCl,???1mol/L HCl?1?,?????????3?? ????????????????????,????60±0.5???????????????,??????,???????????,?????????;??,?????????,??????????
8. (?)?? ????,??Schiff??????40min~60min,??????2~3?,??5min,??????????,??????2???????
(?)??? ??????????,??????????(?????),???0.1%??????????,?????,??????45%?????,????,????????????,???
(?)??????5%????(90?)??15min,???????????(?) (?) (?)?
??????????
1 ????????? ???????,???????????????????,??,?????,????????,????????,?????????,??,??????????????,??????(?????)
10. (3)??:??????????????,???????????????,?????????????,???????(??????)??????
(4)??:????????????,??,??????????? ??,?????????????:????,??????,??????,??1-2??????????????????????,???????????
13. 2 ????????????(?????)
3 ??????????????
???????????????????,?????????????2??4?,????6?????????????????????????
?? :
1)???????????,????????????????????????????,?????????????????,?????????2)??????????????,???????????,???????????????????????3)??,????????????????????????,????????,????????,???????????????
16. ??Feulgen??????????:
1. ??????? ??Feulgen???, ?????????????????????????????????????5%????(90?)??15min,?????????????????
17. 2. ?????? ???????,???????????????????????????????????????????????????,??????????????Feulgen????Bhampy????Helly????Flemming????OsO4????Carnoy????zenker????Bouin-Aller????
????????,?OsO4?Carnoy????,OsO4(1%?0.5%)?Feulgen????????,???OsO4????,??????Carnoy?????Feulgen???,??????Bouin??,????Feulgen????????,??Aller????Bouin-Aller????????
18. 3. ???? Feulgen???????????,???????????????????, ??????;????????????????,??????????,?????????Carnoy????????,???????8~15min ???????????????????(????) ????????????????
4. Schiff????? Feulgen????????????????,??schiff???????????????????,?????????????????????????“DNA?????”
19. ??????????,Schiff???????????DNA?????? ???Schiff????5???????????
5. ???????:
???,???????,????????????,???????SO2?
6. ?????,?????????????,?????????
20.
? ????
1. ??Feulgen ??????????????
2 .Schiff???????????
3. ????????????????????????
????
??Feulgen ????????
??????????