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A. D4. A. pA. SV40. SV-SD4/RRE/SA-pA. RRE. SA5. BPS 1. A4c. BPS 2. A4a. A4b. PPy. PPy. *. *. *. *. *. AAAAAGTGTTGCTTTCATTGCCAAGTTTGTTTCATGACAAAAGCCTTAGGCATCTCCTATGGC AG. SA5. AAAAAGTGTTGCTTTCATTGCCA C GTTTGTTTCATGACAAA C GCCTT C GG CATCTCCTATGGC AG. SA5 AG -.

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  1. A D4 A pA SV40 SV-SD4/RRE/SA-pA RRE SA5 BPS 1 A4c BPS 2 A4a A4b PPy PPy * * * * * AAAAAGTGTTGCTTTCATTGCCAAGTTTGTTTCATGACAAAAGCCTTAGGCATCTCCTATGGCAG SA5 AAAAAGTGTTGCTTTCATTGCCACGTTTGTTTCATGACAAACGCCTTCGGCATCTCCTATGGCAG SA5 AG- AAAAAGTGTGGCGTCCGTTGCCACGTTTGTTTACTAACAAACGCCTTCGGCATCTCCTATGGCAG SA5 b1– b2+AG– AAAAAGTGTGGCGTCCGTTGCCACGTTTGTTTACTAACAAACGCCTTCGGCATCTCCTATTTCAG SA5 b1– b2+AG– Py+ AAAAAGTGTGGCGTCCGTTGCCACGTTTGTTTACTAACAAACGCCCTCGCCTTCTCCTCTTTCAG SA5 b1– b2+AG– Py++ AAAAAGTGTGGCGTCCGTTGCCACGTTTGTTTCATGACAAACGCCCTCGCCTTCTCCTCTTTCAG SA5 b1– AG– Py++ AAAAAGTGTTGCTTTCATTGCCACGTTTGTTTCATGACAAACGCCCTCGCCTTCTCCTCTTTCAG SA5 AG– Py++ AAAAAGTGTTGCTTTCATTGCCAAGTTTGTTTCATGACAAAAGCCCTAGCCTTCTCCTCTTTCAG SA5 Py++ B - Rev + Rev SA5 b1-b2+AG-Py++ SA5 b1-b2+AG-Py++ SA5 b1-b2+AG-Py+ SA5 b1-b2+AG-Py+ SA5 b1-AG-Py++ SA5 b1-AG-Py++ SA5 b1-b2+AG- SA5 b1-b2+AG- SA5 AG-Py++ SA5 AG-Py++ SA5 AG- SA5 Py++ SA5 Py++ SA5 AG- SA5 SA5 us s hGH 1 7' 8' 2 3 4 5 6 7 8 1' 2' 3' 4' 5' 6' C 100 100 80 80 60 60 rel. amount of unspliced p(A)+RNA [%] rel. amount of spliced p(A)+RNA [%] 40 40 20 20 0 0 1' 2' 3' 4' 5' 6' 7' 8' 1 2 3 4 5 6 7 8 Exon 1 Exon 2

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