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Cells Part 3

Cells Part 3. DNA Replication. DNA helices begin unwinding from the nucleosomes Helicase untwists the double helix and exposes complementary strands The site of replication is the replication bubble Each nucleotide strand serves as a template for building a new complementary strand.

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Cells Part 3

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  1. Cells Part 3

  2. DNA Replication • DNA helices begin unwinding from the nucleosomes • Helicase untwists the double helix and exposes complementary strands • The site of replication is the replication bubble • Each nucleotide strand serves as a template for building a new complementary strand

  3. DNA Replication • The replisome uses RNA primers to begin DNA synthesis • DNA polymerase III continues from the primer and covalently adds complementary nucleotides to the template PLAY DNA Replication

  4. DNA Replication • Since DNA polymerase only works in one direction: • A continuous leading strand is synthesized • A discontinuous lagging strand is synthesized • DNA ligase splices together the short segments of the discontinuous strand • Two new telomeres are also synthesized • This process is called semiconservative replication

  5. DNA Replication Figure 3.31

  6. Cell Division • Essential for body growth and tissue repair • Mitosis – nuclear division • Cytokinesis – division of the cytoplasm PLAY Mitosis

  7. Mitosis • The phases of mitosis are: • Prophase • Metaphase • Anaphase • Telophase

  8. Cytokinesis • Cleavage furrow formed in late anaphase by contractile ring • Cytoplasm is pinched into two parts after mitosis ends

  9. Early and Late Prophase • Asters are seen as chromatin condenses into chromosomes • Nucleoli disappear • Centriole pairs separate and the mitotic spindle is formed

  10. Early Prophase Figure 3.32.2

  11. Late Prophase Figure 3.32.3

  12. Metaphase • Chromosomes cluster at the middle of the cell with their centromeres aligned at the exact center, or equator, of the cell • This arrangement of chromosomes along a plane midway between the poles is called the metaphase plate

  13. Metaphase Figure 3.32.4

  14. Anaphase • Centromeres of the chromosomes split • Motor proteins in kinetochores pull chromosomes toward poles

  15. Anaphase Figure 3.32.5

  16. Telophase and Cytokinesis • New sets of chromosomes extend into chromatin • New nuclear membrane is formed from the rough ER • Nucleoli reappear • Generally cytokinesis completes cell division

  17. Telophase and Cytokinesis Figure 3.32.6

  18. Control of Cell Division • Surface-to-volume ratio of cells • Chemical signals such as growth factors and hormones • Contact inhibition • Cyclins and cyclin-dependent kinases (Cdks) complexes

  19. Protein Synthesis • DNA serves as master blueprint for protein synthesis • Genes are segments of DNA carrying instructions for a polypeptide chain • Triplets of nucleotide bases form the genetic library • Each triplet specifies coding for an amino acid

  20. From DNA to Protein Nuclear envelope DNA Transcription Pre-mRNA RNA Processing mRNA Ribosome Translation Polypeptide Figure 3.33

  21. From DNA to Protein DNA Figure 3.33

  22. From DNA to Protein DNA Transcription Figure 3.33

  23. From DNA to Protein DNA Transcription Pre-mRNA RNA Processing mRNA Figure 3.33

  24. From DNA to Protein Nuclear envelope DNA Transcription Pre-mRNA RNA Processing mRNA Figure 3.33

  25. From DNA to Protein Nuclear envelope DNA Transcription Pre-mRNA RNA Processing mRNA Ribosome Translation Polypeptide Figure 3.33

  26. Roles of the Three Types of RNA • Messenger RNA (mRNA) – carries the genetic information from DNA in the nucleus to the ribosomes in the cytoplasm • Transfer RNAs (tRNAs) – bound to amino acids base pair with the codons of mRNA at the ribosome to begin the process of protein synthesis • Ribosomal RNA (rRNA) – a structural component of ribosomes

  27. Transcription • Transfer of information from the sense strand of DNA to RNA • Transcription factor • Loosens histones from DNA in the area to be transcribed • Binds to promoter, a DNA sequence specifying the start site of RNA synthesis • Mediates the binding of RNA polymerase to promoter

  28. Transcription: RNA Polymerase • An enzyme that oversees the synthesis of RNA • Unwinds the DNA template • Adds complementary ribonucleoside triphosphates on the DNA template • Joins these RNA nucleotides together • Encodes a termination signal to stop transcription

  29. Coding strand Termination signal Promoter Template strand Transcription unit In a process mediated by a transcription factor, RNA polymerase binds to promoter and unwinds 16–18 base pairs of the DNA template strand RNA polymerase Unwound DNA RNA polymerase bound to promoter RNA nucleotides mRNA synthesis begins mRNA RNA nucleotides RNA polymerase moves down DNA; mRNA elongates RNA polymerase mRNA synthesis is terminated DNA (a) mRNA transcript Coding strand RNA polymerase Unwinding of DNA Rewinding of DNA Template strand RNA nucleotides mRNA RNA-DNA hybrid region (b) Figure 3.34

  30. Coding strand Termination signal Promoter Template strand Transcription unit (a) Coding strand RNA polymerase Unwinding of DNA Rewinding of DNA Template strand RNA nucleotides mRNA RNA-DNA hybrid region (b) Figure 3.34

  31. Coding strand Termination signal Promoter Template strand Transcription unit In a process mediated by a transcription factor, RNA polymerase binds to promoter and unwinds 16–18 base pairs of the DNA template strand RNA polymerase Unwound DNA RNA polymerase bound to promoter (a) Coding strand RNA polymerase Unwinding of DNA Rewinding of DNA Template strand RNA nucleotides mRNA RNA-DNA hybrid region (b) Figure 3.34

  32. Coding strand Termination signal Promoter Template strand Transcription unit In a process mediated by a transcription factor, RNA polymerase binds to promoter and unwinds 16–18 base pairs of the DNA template strand RNA polymerase Unwound DNA RNA polymerase bound to promoter RNA nucleotides mRNA synthesis begins (a) Coding strand RNA polymerase Unwinding of DNA Rewinding of DNA Template strand RNA nucleotides mRNA RNA-DNA hybrid region (b) Figure 3.34

  33. Coding strand Termination signal Promoter Template strand Transcription unit In a process mediated by a transcription factor, RNA polymerase binds to promoter and unwinds 16–18 base pairs of the DNA template strand RNA polymerase Unwound DNA RNA polymerase bound to promoter RNA nucleotides mRNA synthesis begins mRNA (a) Coding strand RNA polymerase Unwinding of DNA Rewinding of DNA Template strand RNA nucleotides mRNA RNA-DNA hybrid region (b) Figure 3.34

  34. Coding strand Termination signal Promoter Template strand Transcription unit In a process mediated by a transcription factor, RNA polymerase binds to promoter and unwinds 16–18 base pairs of the DNA template strand RNA polymerase Unwound DNA RNA polymerase bound to promoter RNA nucleotides mRNA synthesis begins mRNA RNA nucleotides RNA polymerase moves down DNA; mRNA elongates (a) Coding strand RNA polymerase Unwinding of DNA Rewinding of DNA Template strand RNA nucleotides mRNA RNA-DNA hybrid region (b) Figure 3.34

  35. Coding strand Termination signal Promoter Template strand Transcription unit In a process mediated by a transcription factor, RNA polymerase binds to promoter and unwinds 16–18 base pairs of the DNA template strand RNA polymerase Unwound DNA RNA polymerase bound to promoter RNA nucleotides mRNA synthesis begins mRNA RNA nucleotides RNA polymerase moves down DNA; mRNA elongates RNA polymerase mRNA synthesis is terminated DNA (a) mRNA transcript Coding strand RNA polymerase Unwinding of DNA Rewinding of DNA Template strand RNA nucleotides mRNA RNA-DNA hybrid region (b) Figure 3.34

  36. Initiation of Translation • A leader sequence on mRNA attaches to the small subunit of the ribosome • Methionine-charged initiator tRNA binds to the small subunit • The large ribosomal unit now binds to this complex forming a functional ribosome

  37. Nucleus Nuclear membrane RNA polymerase Nuclear pore mRNA Template strand of DNA Amino acids Released mRNA 1 After mRNA processing, mRNA leaves nucleus and attaches to ribosome, and translation begins. tRNA Aminoacyl-tRNA synthetase Small ribosomal subunit Codon 15 Codon 16 Codon 17 Direction of ribosome advance Portion of mRNA already translated tRNA “head” bearing anticodon Large ribosomal subunit Energized by ATP, the correct amino acid is attached to each species of tRNA by aminoacyl-tRNA synthetase enzyme. 2 Incoming aminoacyl- tRNA hydrogen bonds via its anticodon to complementary mRNA sequence (codon) at the A site on the ribosome. 3 As the ribosome moves along the mRNA, a new amino acid is added to the growing protein chain and the tRNA in the A site is translocated to the P site. 4 Once its amino acid is released, tRNA is ratcheted to the E site and then released to reenter the cytoplasmic pool, ready to be recharged with a new amino acid. Figure 3.36

  38. Nucleus Nuclear membrane RNA polymerase Nuclear pore mRNA Template strand of DNA Released mRNA Figure 3.36

  39. Nucleus Nuclear membrane RNA polymerase Nuclear pore mRNA Template strand of DNA Released mRNA 1 After mRNA processing, mRNA leaves nucleus and attaches to ribosome, and translation begins. Small ribosomal subunit Codon 15 Codon 16 Codon 17 Direction of ribosome advance Portion of mRNA already translated Large ribosomal subunit Figure 3.36

  40. Nucleus Nuclear membrane RNA polymerase Nuclear pore mRNA Template strand of DNA Amino acids Released mRNA 1 After mRNA processing, mRNA leaves nucleus and attaches to ribosome, and translation begins. tRNA Aminoacyl-tRNA synthetase Small ribosomal subunit Codon 15 Codon 16 Codon 17 Direction of ribosome advance Portion of mRNA already translated Large ribosomal subunit Energized by ATP, the correct amino acid is attached to each species of tRNA by aminoacyl-tRNA synthetase enzyme. Figure 3.36

  41. Nucleus Nuclear membrane RNA polymerase Nuclear pore mRNA Template strand of DNA Amino acids Released mRNA 1 After mRNA processing, mRNA leaves nucleus and attaches to ribosome, and translation begins. tRNA Aminoacyl-tRNA synthetase Small ribosomal subunit Codon 15 Codon 16 Codon 17 Direction of ribosome advance Portion of mRNA already translated tRNA “head” bearing anticodon Large ribosomal subunit Energized by ATP, the correct amino acid is attached to each species of tRNA by aminoacyl-tRNA synthetase enzyme. 2 Incoming aminoacyl- tRNA hydrogen bonds via its anticodon to complementary mRNA sequence (codon) at the A site on the ribosome. Figure 3.36

  42. Nucleus Nuclear membrane RNA polymerase Nuclear pore mRNA Template strand of DNA Amino acids Released mRNA 1 After mRNA processing, mRNA leaves nucleus and attaches to ribosome, and translation begins. tRNA Aminoacyl-tRNA synthetase Small ribosomal subunit Codon 15 Codon 16 Codon 17 Direction of ribosome advance Portion of mRNA already translated tRNA “head” bearing anticodon Large ribosomal subunit Energized by ATP, the correct amino acid is attached to each species of tRNA by aminoacyl-tRNA synthetase enzyme. 2 Incoming aminoacyl- tRNA hydrogen bonds via its anticodon to complementary mRNA sequence (codon) at the A site on the ribosome. 3 As the ribosome moves along the mRNA, a new amino acid is added to the growing protein chain and the tRNA in the A site is translocated to the P site. Figure 3.36

  43. Nucleus Nuclear membrane RNA polymerase Nuclear pore mRNA Template strand of DNA Amino acids Released mRNA 1 After mRNA processing, mRNA leaves nucleus and attaches to ribosome, and translation begins. tRNA Aminoacyl-tRNA synthetase Small ribosomal subunit Codon 15 Codon 16 Codon 17 Direction of ribosome advance Portion of mRNA already translated tRNA “head” bearing anticodon Large ribosomal subunit Energized by ATP, the correct amino acid is attached to each species of tRNA by aminoacyl-tRNA synthetase enzyme. 2 Incoming aminoacyl- tRNA hydrogen bonds via its anticodon to complementary mRNA sequence (codon) at the A site on the ribosome. 3 As the ribosome moves along the mRNA, a new amino acid is added to the growing protein chain and the tRNA in the A site is translocated to the P site. 4 Once its amino acid is released, tRNA is ratcheted to the E site and then released to reenter the cytoplasmic pool, ready to be recharged with a new amino acid. Figure 3.36

  44. Genetic Code • RNA codons code for amino acids according to a genetic code Figure 3.35

  45. Information Transfer from DNA to RNA • DNA triplets are transcribed into mRNA codons by RNA polymerase • Codons base pair with tRNA anticodons at the ribosomes • Amino acids are peptide bonded at the ribosomes to form polypeptide chains • Start and stop codons are used in initiating and ending translation

  46. Information Transfer from DNA to RNA Figure 3.38

  47. Other Roles of RNA • Antisense RNA – prevents protein-coding RNA from being translated • MicroRNA – small RNAs that interfere with mRNAs made by certain exons • Riboswitches – mRNAs that act as switches regulating protein synthesis in response to environmental conditions

  48. Cytosolic Protein Degradation • Nonfunctional organelle proteins are degraded by lysosomes • Ubiquitin attaches to soluble proteins and they are degraded in proteasomes

  49. Extracellular Materials • Body fluids and cellular secretions • Extracellular matrix

  50. Developmental Aspects of Cells • All cells of the body contain the same DNA but develop into all the specialized cells of the body • Cells in various parts of the embryo are exposed to different chemical signals that channel them into specific developmental pathways

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