10 likes | 102 Views
Seedling. Mature plant. Y. R. H. C. R. S. L. M. F. kD. PA. 54. 54. PP1A. 59. 54. 42. Supplementary Figure 6.
E N D
Seedling Mature plant Y R H C R S L M F kD PA 54 54 PP1A 59 54 42 Supplementary Figure 6 Supplementary figure 6. Ee;CDKF;1 protein expression and phosphorylation in various organs of leafy spurge. Total proteins were phenol-extracted from root (R), hypocotyl (H), and cotyledon (C) of seedlings and root (R), stem (S), leaf (L), meristem (M), and flower (F) of mature plants. A protein extracted from yeast GF2351 harboring pYX-At;CDKF;1 (Y) was used as a size marker for Ee;CDKF;1. These proteins were fractionated by SDS-PAGE, blotted onto membranes, and incubated with PA or PP1A.