Download
1 / 4
40 likes | 164 Views
C-myc paper reviewer’s suggestions. Use additional SERCA inhibitor to show affects on c-myc, egr-1 and MAPKs Look at c-myc and egr-1 at the protein level when induced with Tg. Use U73343 which is the less active analog of the PLC inhibitor U73122 to control for non-specific effects. What now.
E N D
C-myc paper reviewer’s suggestions • Use additional SERCA inhibitor to show affects on c-myc, egr-1 and MAPKs • Look at c-myc and egr-1 at the protein level when induced with Tg. • Use U73343 which is the less active analog of the PLC inhibitor U73122 to control for non-specific effects
What now • Treat Perk+/+ and Perk-/- MEFs with cyclopiazonic acid (CPA) • Look at eIF2a phosphorylation • Assess c-myc and egr-a at RNA and protein level • Treat Perk+/+ and Perk-/- MEF with Tg • Look at c-myc and egr-1 at protein level
UT CPA hr CPA 2hr Tg UT CPA hr CPA 2hr Tg WT KO WT KO WT KO WT WT KO WT KO WT KO WT eIF2a[P] eIF2a Perk+/+ and Perk-/- MEFs treated with 10mM CPA
