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pI = (pk 1 + pk 2 )/2, the pH where the molecule has a net charge of 0. Ninhydrin Test. Ion Exchange Chromatography. Step 1. Separation of Polypeptide Chains. dissociated by exposure to pH extremes, 8 M urea, 6 M guanidinium hydrochloride, or high salt cencentrations.
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pI = (pk1 + pk2)/2, the pH where the molecule has a net charge of 0
Step 1. Separation of Polypeptide Chains dissociated by exposure to pH extremes, 8 M urea, 6 M guanidinium hydrochloride, or high salt cencentrations
Edman degradation phenylisothiocyanate 50 cycles of reaction can be accomplished on 50 pmol (about 0.1 mg)
Carboxypeptidases cleave amino acid residues from the C-termini
Fmoc solid phase peptide synthesis: A Practical Approach Edited by Weng C. Chan and Peter D. White OXFORD UNIVERSITYPRESS 2000