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Plasmid w/ kanamycin resistance (pKAN)

Plasmid w/ kanamycin resistance (pKAN). Restriction enzyme: Hind III (cuts @ bp 234). pKAN = 4194 bp. Restriction enzyme: Bam HI (cuts @ bp 2095). Thousands of plasmids in our samples. K– (uncut plasmid). K+ (digested plasmid). How many plasmid fragments?. How many plasmid fragments?.

malachi-wilcox
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Plasmid w/ kanamycin resistance (pKAN)

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  1. Plasmid w/ kanamycin resistance (pKAN) Restriction enzyme: Hind III (cuts @ bp 234) pKAN = 4194 bp Restriction enzyme: Bam HI (cuts @ bp 2095)

  2. Thousands of plasmids in our samples

  3. K–(uncut plasmid) K+(digested plasmid) How many plasmid fragments? How many plasmid fragments?

  4. We will then micropipette the plasmids

  5. Load the plasmids into an electrophoresis chamber

  6. Connect the electrophoresis to a power supply…DNA has a negative electric charge.

  7. Negative charge How many fragments do you think will come out of the K+ lane? How many fragments do you think will come out of the K- lane? Positive charge

  8. So now what??? • Plasmids have been engineered for human uses. The human gene for insulin (red) can now be added to the plasmid. The bacteria will produce insulin for diabetics!

  9. So now what??? The bacteria with the recombinant DNA replicates, thus passing the insulin gene onto its offspring. Each cell now will produce insulin for humans to harvest and use.

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