240 likes | 253 Views
Molecular Monitoring in CML the process and questions we can answer. Letizia Foroni. Aims. Review laboratory tests used for the diagnosis of CML Review the information provided by PCR Stopping treatment: how can molecular test help to decide. Process of analysis. Sample collection
E N D
Molecular Monitoring in CMLthe process and questions we can answer Letizia Foroni
Aims • Review laboratory tests used for the diagnosis of CML • Review the information provided by PCR • Stopping treatment: how can molecular test help to decide
Process of analysis Sample collection and analysis Sample examination
The first step: look down a microscope! Increased number of one type of cells, the granulocytes o called because they contain a lot of granules!
Other diagnostic tools to confirm the diagnosis? Cytogenetic analysis
Cytogenetics: Philadelphia chromosome Chr 9 Chr 22: Ph chromosome
Other diagnostic tools to confirm the diagnosis? Molecular analysis
Molecular Monitoring post TKI therapy Are the remaining blood cells normal or leukaemic? For this we require a very sensitive methodology: Real Time PCR or Quantitative PCR
BCR-ABL1: FU analysis Real time quantitative PCR
PCR and International Scale Values 0.75 Lab 1 Procedure 1 Reference material Lab 2 Procedure 2 Lab 3 Conversion Factor 0.55 Procedure 3
Effect of Conversion factor/s on tests from different laboratories
When is it important to test? • At diagnosis • Every 3 months for at least the first 2 years (general practice) but to be discussed with your clinical team. • Every 6 months after 2 years.
Monitoring CML summary CAN we stop?
The best method to use to decide when is best to stopWhich and when?
Conclusions • PCR provides the most sensitive method to monitor disease • Samples at 3 months and every three months predict future outcome • Digital PCR is helping to identify patients who can consider stopping therapy