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動物模式核心實驗室

動物模式核心實驗室. 研究技術 / 方法. Tumorgenesis nude mice model Disease animal model Gene therapy model Drug delivery animal model. 蛋白質體核心實驗室. 研究技術 / 方法. 2D gel electrophoresis Protein identification:Tandem mass spectrometry [ESI/Q-TOF, MALDI-Q-TOF]: protein identification

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動物模式核心實驗室

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  1. 動物模式核心實驗室 研究技術/方法 • Tumorgenesis nude mice model • Disease animal model • Gene therapy model • Drug delivery animal model

  2. 蛋白質體核心實驗室 研究技術/方法 • 2D gel electrophoresis • Protein identification:Tandem mass spectrometry [ESI/Q-TOF, MALDI-Q-TOF]: protein identification • Structural proteomics:fluorescence spectrophotometry, gel filtration, site-directed mutagenesis, circular dichrorism • Recombinant protein production • Protein purification:FPLC ,MDLC • Protein-protein interaction: real-Time biomolecule interaction analysis • Protein-protein interaction:yeast two-hybrid system

  3. 細胞分子生物核心實驗室 研究技術/方法 • Laser capture microdissection • Immunohistochemistry • Electron microscope • Cell culture • Western blot • Northern blot • Immunoprecipitation • Transfection: transient, stable • Electrophoretic mobility shift assay (EMSA) • ChIP Chromatin immunoprecipitation (CHIP) • Promoter activity assay • RNAi construct • Confocal microscope • Flow cytometry

  4. 基因體核心實驗室 • Microarray • Real-time PCR • DNA and RNA extraction • Gene subtraction • Serial analysis of gene expression (SAGE) • Genotyping (DNA polymorphism) 研究技術/方法

  5. 生物資訊暨結構生物學 核心實驗室 研究技術/方法 • Microarray data analysis • SNP data analysis • Genome bioinformatics • sequence analysis • Design of primers • Drug design • Theoretical calculation of small molecules and macromolecules • Structural identification of small molecules and macromolecules

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