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Novi Arisman 1 , Takao Yoshimatsu 1 , Mohammad Nakid Dad Khan 1 ,

Porphyra spp. P. yozoensis. Short-neck clam,  Ruditapes philippinarum  culture using Porphyra  spheroplast in closed recirculation system with special reference to water quality monitoring. PS + oligosaccharides. Enzymatic digestion. Removing cell wall, enhance the digestibility.

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Novi Arisman 1 , Takao Yoshimatsu 1 , Mohammad Nakid Dad Khan 1 ,

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  1. Porphyraspp. P. yozoensis Short-neck clam, Ruditapes philippinarum cultureusing Porphyra spheroplast in closed recirculation systemwith special reference to water quality monitoring PS + oligosaccharides Enzymatic digestion Removing cell wall, enhance the digestibility Liquid mixture ETN Centrifugation Novi Arisman1, Takao Yoshimatsu1, Mohammad Nakid Dad Khan1, A.M. Shahabuddin1, Toshiyoshi Araki1 Freeze drying FDS Supernatant Precipitate PS Spray drying SDS 1Graduate School of Bioresources, Mie University, Tsu, Mie 514-8507, Japan What is Porphyra? Objective Porphyra spp., including P. yezoensis as its representative species, is among the nutritious edible seaweeds. It usually to be used as mainly as a luxury food, wrapped around a small handful of rice in sushi. The experiment aimed to clarify the suitability and availability of feeds as a continual development of new culture feed for aquatic animal from Porphya prepared by enzymatic digestion. METHOD How to prepare Porphyra spheroplast (PS)? SDS ETN FDS This three kinds of PS were used as the experimental feed for sort-neck clam, d in the closed recirculation system. • Why: • Good taste & high nutritive value • High demand • Problem: • Highly dependent on live micro algae (diatom: Chaetoceroscalcitrans) – Integrated algal culture facility RESULT Mean of water quality parameter Rearing results of short-neck clam, Ruditapes philippinarum fed with different type of Porphyra spheroplast in closed recirculation system for 30 days Conclusion Reference Porphyra spheroplasthas a great potential opportunity to be implemented as a substitute for live micro algae, base on the growth, survival, and water quality. Special attention for ETN, this type of PS are predominate as a simpler and less consuming time that particularly beneficial in order to maximize cost-effectiveness. Kalla, A., Yoshimatsu, T., Araki, T., Zhang, D. M., Yamamoto, T., & Sakammoto, S. (2008). Use of Porphyra spheroplasts as feed additive for red sea bream. Fisheries Science, 104–108. Kalla, A., Yoshimatsu, T., Higano, J., Umemoto, Y., Sakamoto, S., & Araki, T. (2008). Novel Diet for Short-Neck Clam by Using Porphyra Spheroplasts. Aquaculture Science, 56(3), 465-466 Khan, M. N., Yoshimatsu, T., Kalla, A., Ishihi, Y., Araki, T., & Sakamoto, S. (2009). Effect of Dif ferent Particle Size of Porphyra Spheroplasts Diet for Short-neck Clam. Aquaculture Science, 57(1), 31-38.

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