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Cry3A transgenic, bark beetle resistant spruce

Cry3A transgenic, bark beetle resistant spruce. NAZV project QH71290 2007-2011. Spruce bark beetle (Ips typographus). Bark beetle galleries. Bacillus thuringiensis crystal protein (Cry, δ -endoTOXin). Agrobacterium interaction with the plant cell. Major problems:.

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Cry3A transgenic, bark beetle resistant spruce

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  1. Cry3A transgenic, bark beetle resistant spruce NAZV project QH712902007-2011

  2. Spruce bark beetle (Ips typographus)

  3. Bark beetle galleries

  4. Bacillus thuringiensiscrystal protein (Cry, δ-endoTOXin)

  5. Agrobacterium interaction with the plant cell

  6. Major problems: • Spruce is difficult to transform • No known Bt toxin is toxic enough to bark beetle

  7. Spruce callus cultures

  8. Somatic embryogenesis induction conversion cultivation

  9. Modified Cry3A genes Cry3A orig Synt TOX-1 (602 aa)-codons Picea optimized Ndel/sTOX-1 Cdel/sTOX-1 (602 aa) Cdel/Ndel/sTOX-1 GUS

  10. Refined modification of domain II

  11. Expression vector

  12. PAGE analysis of modified Cry3A expression

  13. WESTERN detection of modified Cry3A proteins

  14. Toxicity tests on live beetles

  15. K o n e c p r e s e n t a c e

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