Bio212 Laboratories 8 & 9 Cat Dissection I & II (Combined Lab; All Systems)

Bio212 Laboratories 8 & 9 Cat Dissection I & II(Combined Lab; All Systems)

Lab Schedule for Rest of Semester • Cat dissection labs • Dissection (today only) • Cats are already prepared for examination • Cardiovascular (Dissec Ex 4 in Wood; Ex 45 in Hole) • Digestive (Disssec Ex 7 in Wood; Ex. 48 in Hole) • Respiratory (Dissec Ex 6 in Wood; Ex. 51 in Hole) • Urinary and Reproductive (Dissec Ex 8/9 in Wood; Ex. 56/59 in Hole) • Use the Instructions handed out today instead of those in your Laboratory Guide • Lab Exam 2 (4/16, MW AM; 4/18, MW PM; 4/20, Friday)

Objectives for Today’s Lab • Dissect the preserved cat • Identify the structures listed on the Cat Dissection Checklist in your Laboratory Guide for Lab 8 AND 9 – we’re doing both today • Don’t remove any organs (other sections will be using these cats • DON’T RUSH YOUR DISSECTION!!!

Precautions • You must wear lab coat, gloves, and goggles for this lab! • ABSOLUTELY NO FOOD OR DRINK • Be EXTREMELY careful when using the scalpels • Keep your index finger on the top of the blade • Be careful not to inadvertently cut structures • Use a blunt probe, or your gloved fingers to dissect away tissue. Use the scalpel ONLY for initial cutting. Proceed S-L-O-W-L-Y. • Discard scraps/liquid into the appropriate container • Gloves, paper towels, plastic bags -> regular trash

Getting started… • Work in groups of 5-6 (there are 4 cats) • You may have either a male or female cat (there should be two of each); note which you have • Get some paper towels and keep them at your lab table • Get a lab coat; when finished, put back in box • Get your instruments and dissecting tray (large trays in the front of the lab) ready

Getting started… • Note that the cats have been dual injected with dyes (red = arteries, blue = veins) • Note the differences in the anatomy of the cat (vs. the human) in the blood vessels around the heart and in the pelvic area (see your Laboratory Guide)

Cardiovascular system • Open the rib cage by cutting just to one side of the sternum with scissors then spread the ribs • Examine the position of the thoracic organs, i.e., heart and lungs and the membranes surrounding them • Gently begin to remove the fatty/glandular tissue from the surface of the heart to expose the vessels around the heart (see preserved cat) • Work S-L-O-W-L-Y to dissect away the CT from the vessels. Use a blunt probe.

Cardiovascular System Dissection • You should be able to locate the vessels listed in your Lab Guide (Cat Dissection Checklist) • You should make a frontal (coronal) section through the heart to expose the chambers • You should be able to recognize/name the chambers of the heart • Notice the pulmonary vessels and try to trace their path to their connections to the lungs

Cardiovascular System • In order to see the vessels that you are responsible for on Cat Dissection Checklist, you will need to • Begin at the heart and work outwards taking care not to destroy any organs we will be examining in the next labs • Look at the vessels in the neck • Look at the vessels in the thorax and abdomen • Look at the vessels in the legs down the first third of the femur • Use the atlases I have placed in the front of the room if you need help in identifying structures/organs

When You Are Done for the Day… • Put any accidentally removed organs back in the cat • Wrap flaps of skin and muscle around the cat to keep it moist, and spray it with Ward’s Safe Preservative that is on the table in the front of the lab (WSP) • Wrap the cat with paper towels and spray the paper towels with WSP until soaked

When You Are Done For the Day • Place the cat into a new plastic bag, remove as much air as you can, and seal it tightly with the bag sealer. • Then put it in another bag and seal it tightly with the bag sealer. When you are done, PLEASE SHOW ME!!! • Tag your cat/bag and write “Dr. Erianne” in pencil on tag • Place the bag in the white plastic tray • Wash all instruments THOROUGHLY and replace them in tray.

Bio212 Laboratories 8 & 9 Cat Dissection I & II (Combined Lab; All Systems)