1 / 10

Project Scope Shootout

Slide = Chroma Full Skin Section Nuclei stained with Hoechts F- Actin - Alexa 488 Elastin - Cy3 Collegen - Cy5. Project Scope Shootout. Stella Breslin Biosc 3 / Cohort 3 Spring 2010. Chroma Leica Sp5 5/4/10.

naasir
Download Presentation

Project Scope Shootout

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Slide = Chroma Full Skin Section Nuclei stained with Hoechts F-Actin - Alexa 488 Elastin - Cy3 Collegen - Cy5 Project Scope Shootout Stella Breslin Biosc 3 / Cohort 3 Spring 2010

  2. ChromaLeica Sp5 5/4/10 5/4/10 SBChromaMay4.lif/Image051 mag: 20x Plan Apo 2channels 512x512 AOTF 30% (514) PMT2 519nm - 630nm off 0 HV 827 / AOTF (514) 20% PMT3 638nm - 765nm off -1 HV 750 / line avg 1 frame avg 3 pinhole 30.3 µm pinhole airy 0.5

  3. Leica Spinning Disk 5/15/10 5/15/10 ChromaSD.lif/Series22 HC PL FLUOTAR 10.0x0.30 DRY 3 Channels 1 frame Exp G 932 ms, Red 932 ms, Blue 1000 ms Gain: G 5.9 R 5.9 B 6.3

  4. Nikon 20x 5/8/10 Nikon 3 5/8/10 Mag: 20x Exp: TRITC 200ms FITC 500ms DAPI 52ms Contrast Stretch ImageJ

  5. Zeiss 2 / DAPI Filter Added 5/11/10 ChromaSlide Multichannel mag: 20x Exp: FITC 346ms Rhod 286ms DAPI 145ms Br -.057,-0.57-,0.66 Con 1.19, 1.06, 1.37 gamma 1.0

  6. Zeiss 2 / Darkfield 5/11/10 ChromaSlide Darkfield mag: 20x Exp: 130ms Br -0.03 Con 1.6 gamma 1.0

  7. Chroma -Zeiss 2 / Rhod/FITC/DAPI/DIC overlay - 5/11/10 ChromaSlide MC Overlay Mag: 40x Exp: FITC 598ms Rhod 286ms DAPI 24ms Con 1.0 gamma 1.0 DIC exp 4ms / Contrast stretch each channel and DIC overlay done in Adobe PS

  8. Chroma -Olympus 3 - 5/22/10 ChromaSlide Multichannel mag: 10x MC Exp: Red 518.5ms Green 441.9 Offset R -894 G-662 Gain 1.0 Color Merge Contrast Stretch in Adobe PS

  9. Chroma - Olympus 3- 5/22/10 5/22/10 ChromaSlide mag : 10x Phase exp: 18.6 ms gain 1.0 offset 0 Contrast Stretch Image J

  10. Slide 6: your succinct comments comparing the images acquired on Comparisons

More Related