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B. MALDI IMS Ischemic Hemisphere

Figure 1. MALDI IMS Control Hemisphere. B. MALDI IMS Ischemic Hemisphere. m/z 760.6. m/z 760.6. 300. 300. 400. 500. 600. 700. 800. 900. 1000. Figure 2. 130. A. Total ions (Control Hippocampus). 760.6. 798.6. 548.5. 782.6. m/z 760.6. Signal Intensity. 1500. 548.5.

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B. MALDI IMS Ischemic Hemisphere

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  1. Figure 1 • MALDI IMS • Control Hemisphere B. MALDI IMS Ischemic Hemisphere m/z 760.6 m/z 760.6 300 300

  2. 400 500 600 700 800 900 1000 Figure 2 130 A. Total ions (Control Hippocampus) 760.6 798.6 548.5 782.6 m/z 760.6 Signal Intensity 1500 548.5 170 B. Ischemic Hippocampus 760.6 782.6 798.6 8x m/z 760.6 Signal Intensity m/z

  3. Figure 3 • MALDI IMS • Control Hemisphere B. MALDI IMS Ischemic Hemisphere m/z 548.5 m/z 548.5 1500 1500

  4. Figure 4 • TTC Stained • Rat Brain Slice B. 16:0/18:1 PC [M+H]+ m/z 760.6 550 D. 16:0/18:1 PC [M+K]+ m/z 798.6 C. 16:0/18:1 PC [M+Na]+ m/z 782.6 1400 2300

  5. Figure 5 A. MALDI IMS of Rat Brain 96 hours post TBI B. CID (pos ion) m/z 548.5 m/z 548.5 264.3 100 548.5 200 530.5 Relative Intensity 282.3 308.3 252.3 100 200 300 400 500 600 m/z

  6. 100 200 300 400 500 600 m/z Supplemental Figure 1 [Cer(d18:1/13C4-16:0)-H2O]+ 264.3 CID m/z 524.5 Cer 18:1/13C4-16:0 [M+H]+ -H2O 100 ESI/MS 524.5 POPC-Na+ POPC-H+ Cer(d18:1/13C-16)+ 782.6 760.6 Cer(d18:1/13C-16)-H2O+ 723.5 564.5 577.5 524.5 Relative Intensity 400 600 800 m/z 506.6 282.3 252.3 494.5

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