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EGF 100ng/ml 10min

Suppl. F. Vec ShcC. EGF 100ng/ml 10min. - + - +. WB: a pTyr. Phosphorylation of ShcA by EGF is suppressed in the cells transiently expressing ShcC.

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EGF 100ng/ml 10min

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  1. Suppl. F Vec ShcC EGF 100ng/ml 10min - + - + WB: a pTyr Phosphorylation of ShcA by EGF is suppressed in the cells transiently expressing ShcC. Overexpression ShcC suppressed ShcA phosphorylation under the stimulation of EGF even in the transient expression of the ShcC-expressing vector. The result was consistent with the analysis using ShcC expressing clones. ShcA IP: aShcA a ShcA aShcC a pTyr whole EGFR aEGFR

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