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Lesion3 ( Les3 )

Lesion3 ( Les3 ). By Jessica Johns & Erika Bertelsen. Lesion3 ( Les3 ) JLJ, EMB, 1-13-05. Some lesions on a leaf from a Les3 mutant plant. Lesion3 , what is it?.

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Lesion3 ( Les3 )

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  1. Lesion3 (Les3) By Jessica Johns & Erika Bertelsen Lesion3 (Les3) JLJ, EMB, 1-13-05

  2. Some lesions on a leaf from a Les3 mutant plant Lesion3, what is it? Lesion3 is a dominant genetic mutation in which dead spots (lesions) appear on the leaves of corn plants. The lesions do not appear on the leaves for about six weeks.

  3. What Causes a Lesion? A lesion is caused by a genetic mutation. The basic pattern of DNA is coded with the nucleotides that are lettered A, T, G, and C. A is always base-paired with T G is always base-paired with C When there is a malfunction or skip in the Les2 gene sequence it causes a mutation. The genetic mutation then causes a physical mutation which is expressed as a visible lesion (phenotype) on the plants leaves.

  4. OurQuestion When we first planted our seedlings we asked ourselves two questions, how many of our plants would get lesions, and just how long our plants would survive once they developed lesions. As we observed our plants over the weeks we found the answer.

  5. Our Observations Week 1Light green stems, small leaves, and no lesions. Week 2Bottom leaves are a darker green, while the upper leaves are still light. The stems are purplish-red, and there are slight lesions. Week 3Plants are now large. No new lesions. Week 4Twelve out of the sixteen plants have developed lesions. The other four are wild-type* in appearance. * A wild-type is a normal-looking plant which shows no mutation.

  6. Why are there wild types? Lesion mutant: Ll or LL Normal: ll Test-cross: Parents: (P1) Ll x ll Offspring: (F1) Ll ll Ll ll Notice that not all have lesions

  7. Field Pictures of Les3 lesions

  8. Our Answer Only 12 of our 16 plants showed lesions. The lesions which developed were not severe, and we didn’t think they would affect the plants health. Our plants which got lesions did not die, but they did become sick and in time they may well die.

  9. Reference 1976 RB Ashman & AJ Ullsturp A Heritable, Noninfectious Necrosis of Maize Leaves. Journal of Heredity Volume 67 Pages 220-222

  10. Field placard Name: Lesion3 (Les3) Locus: Chromosome 10 (linkage map bin 10.06) Field placard by:Jessica Johns & Erika Bertelsen Middle School Mentorship, Fall 2004 School of Arts and SciencesLeon County Charter SchoolTallahassee, FL

  11. A special thanks We would like to offer a special thanks to… Dr. Hank Bass Ms. Debbie Figueroa Ms. Bobbye Hill Dr. Brian Ring And of course our wonderful Ms. Crow

  12. The end

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