1 / 16

Protective effect of melatonin on thymus of rats exposed to microwave radiation

Branka Djordjevic, Dusan Sokolovic, Andrej Veljkovic , Milena Marinkovic, Jelena Basic , Gordana Kocic , Jelenka Nikolic Department of Biochemistry, Faculty of Medicine , University of Nis. Bul. dr Zorana Djindjica 81, 18000 Nis, Serbia.

nell-morse
Download Presentation

Protective effect of melatonin on thymus of rats exposed to microwave radiation

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Branka Djordjevic, Dusan Sokolovic, Andrej Veljkovic, Milena Marinkovic, Jelena Basic, Gordana Kocic, Jelenka Nikolic Department of Biochemistry, Faculty of Medicine, University of Nis. Bul. dr Zorana Djindjica 81, 18000 Nis, Serbia Protective effect of melatonin on thymus of rats exposed to microwave radiation

  2. Microwaves 300 GHz - 300 MHz 1mm-1m 1.24 µeV - 1.24 meV

  3. Microwaves and oxidative stress

  4. “Thymus plays an important role in the immune system development...” Matsui N, Nitta T, Takahama Y. Development of the thymus and immune system. Brain Nerve 2011;63(7):679-84. “...which can be modulated by numerous environmental factors, including MWs.” Quaglino D, Capri M, Ronchetti IP. Modulation of cell death in the rat thymus. Light and electron microscopic investigations. Ann N Y Acad Sci 2000;926:79-82. “Oxidative stress has been implicated in thymus disorders.“ Yan M, Shen J, Person MD, Kuang X, Lynn WS, Atlas D, et al. Endoplasmic reticulum stress and unfolded protein response in Atm-deficient thymocytes and thymic lymphoma cells are attributable to oxidative stress. Neoplasia. 2008;10(2):160-7.

  5. MWs have already been linked to cancer, especially childhood leukemia and T cell non Hodgkin lymphoma. Myung SK, Ju W, McDonnell DD, Lee YJ, Kazinets G, Cheng CT, et al. Mobile phone use and risk of tumors: a meta-analysis. J Clin Oncol 2009;27(33):5565-72. Lowenthal RM, Tuck DM, Bray IC. Residential exposure to electric power transmission lines and risk of lymphoproliferative and myeloproliferative disorders: a case-control study. Intern Med J 2007; 37(9):614-9.

  6. Melatonin(N-acetyl-5-methoxytryptamine) sinthetised and released from pineal gland MT1 and MT2 receptors expressed in all lymphocyte subpopulations immunostimulant and a powerfull antioxidant directly neutralizes free radicals stimulates antioxidant enzyme activity prevents oxidative injury of DNA molecules inhibits apoptosis in immune cells

  7. Aim of the study • The present study was designed to evaluate the effects of MWs on oxidative stress parametars in the thymus tissue and • to clarify if melatonin treatment may favorably impact on thymus tissue injury in rats exposed to microwave radiation.

  8. Experimental setup • 3-week dailyadministration ofmelatonin (2 mg/kg i.p.) • adult Wistar rats • exposition to microwaves (4 hours/day) • Wistar rats were divided in four groups: • I (control) - rats treated with saline, • II (Mel) - rats treated with melatonin, • III (MWs) - microwave exposed rats, • IV (MWs + Mel) - MWs exposed rats treated with melatonin.

  9. Exposure system • plastic cage • mobile phone (NOKIA 3110 classic, Nokia Mobile Phones Ltd.) • 900 MHz electromagnetic near field continuous wave • AARONIA SPECTRAN HF6080 EMF meter • SAR0.089 W/kg (0.043-0.135 W/kg)

  10. Biochemical analysis • Thymus tissue was prepared as 10% homogenate. • Malondialdehyde (MDA) • Protein carbonyls • Catalase (CAT) • Xantine oxidase (XO) • Proteins

  11. MDA *** (p < 0.001) + (p < 0.05) Ohkawa H, OhishiN, Yagi K.Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95(2):351-8.

  12. Protein carbonyls *** (p < 0.001) +++ (p < 0.001) Levine RL, WIilliams JA, Stadtman ER, et al. Carbonyl assay for determination of oxidatively modified proteins. Methods Enzymol 1994;233:346-57.

  13. Catalase *** (p < 0.001) +++ (p < 0.001) Goth L . A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta 1991;196(2-3):143-51.

  14. Xantine oxidase *** (p < 0.001) +++ (p < 0.001) Hashimoto S. A new spectrophotometric assay method of xanthine oxidase in crude tissue homogenate. Anal Biochem 1974;62(2):426-35.

  15. Conclusion • Melatonin exerts potent antioxidant effects in thymus of rats exposed to microwaves.

  16. This work was supported by the Ministry of Education and Science of Republic Serbia (Project 43012). Branka Djordjevic, Dusan Sokolovic, Andrej Veljkovic, Milena Marinkovic, Jelena Basic, Gordana Kocic, Jelenka Nikolic Department of Biochemistry, Faculty of Medicine, University of Nis. Thank you very much for your kind attention!

More Related