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PAMC: Polar and Alpine Microbial Collection

PAMC: Polar and Alpine Microbial Collection. Yung Mi Lee 1 , Cheng- Dae Choe 2 , Jeong -Han Yim 1 , Hong Kum Lee 1 , and Soon Gyu Hong 1 1 Korea Polar Research Institute 2 BRN Science Co. Needs for Database. ▣ Management and sharing of bio-resources from polar areas

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PAMC: Polar and Alpine Microbial Collection

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  1. PAMC: Polar and Alpine Microbial Collection Yung Mi Lee1, Cheng-Dae Choe2, Jeong-Han Yim1, Hong Kum Lee1, and Soon Gyu Hong1 1 Korea Polar Research Institute 2 BRN Science Co.

  2. Needs for Database ▣ Management and sharing of bio-resources from polar areas ▣ Sharing biodiversity information with national and international organization www. iobis.org

  3. Culture Collections 755,818 of them are Bacteria. 505,521 of them are Fungi. . . . www.wfcc.info

  4. PAMC: Polar and Alpine Microbial Collection ▣pamc.kopri.re.kr ▣ Culture Collection to maintain and distribute microorganisms originated from polar areas ▣ Approximately 500 strains are available for delivery and the number will increase gradually v Polar and Alpine Microbial Collection Polar and Alpine Microbial Collection About PAMC

  5. Data Management of PAMC Public databases • Metadata upload (AMD) • Original data transfer (EBA, SCAR-Marbin etc) • Data download (GenBank Taxonomy etc) Search Deposit of resources PAMC Public users Contributors Public users Data download Delivery of resources Curators • Long-term preservation of bio-resources • Identification • Characterization • Data updates

  6. Strains ▣ Microbial cultures • Bacteria: 6,400 strains • Fungi: 300 strains ▣ Preservation • Freezing using Glycerol (-180C) and DMSO (-80C) • Freeze-dry (4C) Preservation of strains

  7. Summary of PAMC Strains Habitats of strains Locality distribution of strains Phylum composition of identified strains Actinobacteria, 188 Bacteroidetes,191 Gammaproteobacteria,597 Firmicutes, 265 Betaproteobacteria,49 Alphaproteobacteria,50

  8. Composition of Identified Strains Firmicutes 11 Genera 265 Strains Actinobacteria 16 Genera 188 Strains Bacteroidetes 25 Genera 191 Strains Betaproteobacteria 4 Genera 49 Strains Gammaproteobacteria 18 Genera 597 Strains Alphaproteobacteria 16 Genera 50 Strains

  9. Physiological Characteristics No. of Strains ▲ Growth on the 96 well plate Growth Temperature No. of Strains ▲ Formation of hydrolytic zone on the 96 well plate Protease Lipase Exopolysaccharide

  10. Sample Database

  11. Strain Database

  12. PAMC - Searching ① ② Pseudoalteromonas ③ v v v ④ v

  13. PAMC – Advanced Searching Polar and Alpine Microbial Collection Polar and Alpine Microbial Collection

  14. Searching Keywords in PAMC Polar and Alpine Microbial Collection Taxonomy Geography Habitats Characteristics Searching for microbial strains using various keywords such as taxonomy, geography, habitatsand physiological characteristics are available in PAMC.

  15. Vision of PAMC ▣ To maintain strains for the sustainable use ▣ To supply authentic strains for polar sciences ▣ To expand the service materials: fungal culture, gDNA, metabolite extract, etc ▣ PAMC website will be available on 1 October

  16. Thank you!

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