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MSU 300

Tunica. Tunica. Tunica. Tunica. Supplementary Figure 1. ER stress-induced secretion of mature IL-1β by THP-1 cells is dependent on the NLRP3 inflammasome. MSU 300. MSU 300. MSU 300. MSU 300. R837 15. 10. 15. 20. R837 15. 10. 15. 20. R837 15. 10. 15. 20. R837 15. 10. 15. 20.

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MSU 300

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  1. Tunica Tunica Tunica Tunica Supplementary Figure 1. ER stress-induced secretion of mature IL-1β by THP-1 cells is dependent on the NLRP3 inflammasome. MSU 300 MSU 300 MSU 300 MSU 300 R837 15 10 15 20 R837 15 10 15 20 R837 15 10 15 20 R837 15 10 15 20 Mock Mock Mock Mock µg/ml SN xT shRNA : Scramble NLRP3 ASC Caspase-1 CHOP IL-1 pIL-1 pCasp1 ASC NLRP3 Sup. Fig. 1

  2. Supplementary Figure 2. ER stress-induced activation of the NLRP3 inflammasome is independent of TLR and RLR signaling. BFA 0.1 BFA 0.1 A R837 15 R837 15 mock Tunica 15 mock Tunica 15 µg/ml SN IL-1 pIL-1 IL-1 pCasp1 MyD88 pCasp1 pIL-1 Casp1 pCasp1 pIL-1 CHOP xT Scramble MyD88 shRNA : MSU 150 µg/ml MSU 150 µg/ml MSU 150 µg/ml Tunica 10 µg/ml Tunica 20 µg/ml Tunica 10 µg/ml Tunica 20 µg/ml MSU 150 µg/ml Thapsi 5 µM Thapsi 10 µM Thapsi 5 µM Thapsi 10 µM B C pIL-1 CHOP pCasp1 Casp1 pIL-1 pCasp1 IL-1 Tunica 10 µg/ml Tunica 20 µg/ml Tunica 10 µg/ml Tunica 20 µg/ml Thapsi 5 µM Thapsi 10 µM Thapsi 5 µM Thapsi 10 µM Mock Mock Mock Mock SN SN xT xT Mavs+/+ Mavs-/- Trif+/+ Trif-/- Sup. Fig. 2

  3. Supplementary Figure 3. IRE1α shutdown in shRNA-expressing THP-1 cells. Empty vector shIRE1 #1 shIRE1 #2 shIRE1 #3 IRE1α Tubulin xT Sup. Fig. 3

  4. Supplementary Figure 4. TRAF2 and ASK1 are dispensable for ER stress-induced NLRP3 inflammasome activation. MSU 300 µg/ml R837 20 µg/ml MSU 300 µg/ml R837 20 µg/ml MSU 300 µg/ml R837 20 µg/ml Tunica 10 µg/ml Tunica 10 µg/ml Tunica 10 µg/ml A Mock Mock Mock TRAF2 IL-1 pIL-1 SN xT shRNA : Scramble TRAF2 #1 TRAF2 #2 Tunica 10 µg/ml Tunica 10 µg/ml Tunica 5 µg/ml Tunica 5 µg/ml Thapsi 2.5 µM Thapsi 2.5 µM Thapsi 5 µM Thapsi 5 µM MSU 300 µg/ml MSU 300 µg/ml B Mock Mock pCasp1 pCasp1 pIL-1 Casp1 SN xT Ask1+/+ Ask1-/- Sup. Fig. 4

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