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Reviewing Molecular Biology

Reviewing Molecular Biology. AP Biology Chapters 16,17 ,18 & 20. With the cell type you are assigned:. Identify 3 genes that could be on in that cell type and 3 genes that could be off and be able to explain why this might be true!

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Reviewing Molecular Biology

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  1. Reviewing Molecular Biology AP Biology Chapters 16,17 ,18 & 20

  2. With the cell type you are assigned: Identify 3 genes that could be on in that cell type and 3 genes that could be off and be able to explain why this might be true! Describe the different levels of gene regulation and why gene regulation may be controlled at more than one level. Identify and diagram on your paper 4 ways to activate gene expression (turn genes on) and 4 ways to inhibit gene expression (turn genes off)

  3. Examples Myoblast Bone Marrow Cell Metastatic Cancer Cell

  4. With the biotechnology you are assigned: What type of organism was the enzyme or process used in? How and why is the enzyme used in biotechnology? What can we apply this biotechnology to study?

  5. Borrowed Biotech RNAi Genetically modifying a bacteria PCR Cutting up a gene to run a gel

  6. Plasmid cloning AP Biology 2007 How do we construct a plasmid from a restriction map?

  7. Plasmid Map

  8. Ways to identify that a gene has been inserted into a plasmid: Connect the gene to a fluorescent protein like GFP! Blue White Screen

  9. Blue White Screen

  10. Challenges to cloning eukaryotic organisms (GMO’s): • What are some challenges to cloning? • From Embryos • From Adult cells • With cloning for therapeutic purposes • With cloning for agricultural purposes • http://www.pbs.org/wgbh/harvest/etc/video.html

  11. How do we know all this information about DNA? • Human Genome Project • Basic Science: • How do you sequence a small of DNA? • http://www.pbs.org/wgbh/nova/body/sequence-DNA-for-yourself.html • How did we get to the entire genome sequence? • Public Genome Project: from NOVA • http://www.pbs.org/wgbh/nova/body/public-genomes.html • Is personalized, genetic medicine in our future? • Personal Genome Project: from NOVA • http://www.pbs.org/wgbh/nova/body/church-genome-vid.html

  12. What types of questions could make up a test on DNA and Gene Regulation? Test 3/13!! What types of things would lend well to multiple choice? What types of things could fit in best in the experimental/problem solving section? What types of questions would lend well to short answer? What types of questions would be best suited for open response?

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