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Lesson Overview

Lesson Overview. 12.3 DNA Replication. Copying the Code. What role does DNA polymerase play in copying DNA? ________________ is an enzyme that joins individual nucleotides to produce a new strand of DNA. Copying the Code.

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Lesson Overview

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  1. Lesson Overview 12.3 DNA Replication

  2. Copying the Code • What role does DNA polymerase play in copying DNA? • ________________ is an enzyme that joins individual nucleotides to produce a new strand of DNA.

  3. Copying the Code • ______________in the double helix explained how DNA could be copied, or replicated, because each base on one strand pairs with only one base on the opposite strand. • _______________of the double helix has all the information needed to reconstruct the other half by the mechanism of base pairing. • Because each strand can be used to make the other strand, the strands are said to be _________________.

  4. The Replication Process • Before a cell divides, it duplicates its DNA in a copying process called _______________. • This process ensures that each resulting cell has the ___________ ______________ of DNA molecules.

  5. The Replication Process • During replication, the DNA molecule separates into two strands and then produces _________________strands following the rules of base pairing. • Each strand of the double helix of DNA serves as a ______________ , or model, for the new strand.

  6. The Replication Process • The two strands of the double helix separate, or “___________,” allowing two replication forks to form.

  7. The Replication Process • As each new strand forms, new bases are added following the ________________________. • If the base on the old strand is adenine, then _____________ is added to the newly forming strand. • Likewise, guanine is always paired to _______________.

  8. The Replication Process • The result of replication is two DNA molecules ______________to each other and to the original molecule. • Each DNA molecule resulting from replication has one ___________ strand and one _______ strand.

  9. The Role of Enzymes • DNA replication is carried out by a series of _________. They first “______” a molecule of DNA by breaking the ___________ bonds between base pairs and ________________ the two strands of the molecule. • Each strand then serves as a _______________ for the attachment of complementary bases.

  10. The Role of Enzymes • The principal enzyme involved in DNA replication is called _____ _______________________. • DNA polymerase is an enzyme that joins __________________ to produce a new strand of DNA. • DNA polymerase also “_______________” each new DNA strand, ensuring that each molecule is a perfect copy of the original.

  11. Telomeres • The tips of chromosomes are known as ____________. • The ends of DNA molecules, located at the telomeres, are particularly____________________________. • Over time, DNA may actually be _________ from telomeres each time a chromosome is _____________. • An enzyme called ______________compensates for this problem by adding short, repeated DNA sequences to telomeres, lengthening the chromosomes slightly and making it less likely that important gene sequences will be lost from the telomeres during replication.

  12. Replication in Living Cells • How does DNA replication differ in prokaryotic cells and eukaryotic cells? • Replication in most prokaryotic cells starts from a ____________and proceeds in ______directions until the entire chromosome is copied.

  13. Replication in Living Cells • How does DNA replication differ in prokaryotic cells and eukaryotic cells? • In eukaryotic cells, replication may begin at ___________or even __________ of places on the DNA molecule, proceeding in _________ directions until each chromosome is completely copied.

  14. Replication in Living Cells • The cells of most prokaryotes have a ____________________ molecule in the cytoplasm, containing nearly all the cell’s genetic information. • Eukaryotic cells, on the other hand, can have up to ______________DNA. Nearly all of the DNA of eukaryotic cells is found in the ________________.

  15. Prokaryotic DNA Replication • In most prokaryotes, DNA replication does not start until ________________ bind to a single starting point on the chromosome. This triggers the beginning of DNA replication. • Replication in most prokaryotic cells starts from a ________ and proceeds in two directions until the entire chromosome is copied.

  16. Eukaryotic DNA Replication • Eukaryotic chromosomes are generally much ________ than those of prokaryotes. • In eukaryotic cells, replication may begin at _________or even _____________ of places on the DNA molecule, proceeding in both directions until each chromosome is completely copied.

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