1 / 12

Leipzig, 23-25 April 2002 INRA, Avignon, France V. Chovelon, research scientist

Garlic and Health, P9 report. Leipzig, 23-25 April 2002 INRA, Avignon, France V. Chovelon, research scientist JP Leroux, research technician F. Mirrione, temporary agent (April 02). The objectives. Somatic embryogenesis plant regeneration and acclimatisation culture and evaluation

papina
Download Presentation

Leipzig, 23-25 April 2002 INRA, Avignon, France V. Chovelon, research scientist

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Garlic and Health, P9 report Leipzig, 23-25 April 2002 • INRA, Avignon, France • V. Chovelon, research scientist • JP Leroux, research technician • F. Mirrione, temporary agent (April 02)

  2. The objectives • Somatic embryogenesis • plant regeneration and acclimatisation • culture and evaluation • sanitary aspects • production of virus-free bulbs • meristem culture

  3. Somatic embryogenesis • milestones • Rouge Reunion, Messidrôme, Morasol: 200 plants from callus and 200 plants from cell suspension • in vitro culture, acclimatisation • field culture, evaluation • flow cytometry (P8), AFLP analyses (P1), • biochemistry (P5) • morphological and physiological notations (P8,P9) • deliverables • paper on regeneration of garlic plants issued from embryogenic callus (done) • paper on regeneration and acclimatisation of garlic plants issued from cell suspension (in progress)

  4. Rouge Reunion • plants from callus and cell suspensions • culture in tunnel • AFLP analyses (P1) • flow cytometry • phenotypic notations • harvest at maturity - field evaluation at Reunion Island (P8)

  5. In vitro culture Morasol plants from callus or cell suspension

  6. In vitro culture • Messidrôme plants issued from callus

  7. Acclimatisation and field culture • Morasol plants from callus and cell suspension

  8. Acclimatisation and field culture • Messidrôme plants issued from callus

  9. In vitro bulb production • factors for bulb induction and enlargement - Morasol, Messidrôme, Printanor: plants issued from cell suspensions - saccharose concentration: 40-60g/l - cold treatment: 5 weeks at 5°C - quality of light: additional far-red light - photoperiod: 12 or 16h - temperature: 22°C/night - 24°C/day

  10. Sanitary aspects • production of virus-free plants • 300 plants/variety • Messidrôme, Morasol • Printanor, Rouge Reunion • virological control • all the plants are potyvirus-free • some one contained latent viruses • Messidrôme: 100% GCLV, 34% MbFV • Morasol: 5.6% GCLV, 83% MbFV • Printanor: 100% MbFV • alliin analyses

  11. Sanitary aspects • virus elimination by meristem culture

  12. Workplan for 2002-03 • Embryogenesis, genetic characterisation • Rouge Reunion • field evaluation (P8), biochemistry (P5) • Messidrôme, Morasol from callus • field culture (P9), flow cytometry (P8), AFLP (P1) • Printanor from callus • in vitro culture, acclimatisation • Messidrôme, Morasol and Printanor from cell suspension • in vitro bulbs production • Sanitary aspects • production of virus-free bulbs (P9) • meristem culture (P9) acclimatisation, field culture

More Related