DO NOW: Take out your microscope YELLOW packets from last week.

1. DO NOW: Take out your microscope YELLOW packets from last week. In your NOTES section of your binder, please list EIGHT differences between these two pictures.

3. Stare at the red dot on the girl’s nose for 30 seconds. Turn your eyes to a plain surface. Blink repeatedly and quickly.

9. Using a Compound Light Microscope Directed Reading

10. Using the Microscope ocular lens 10 1.) The magnifies the image x. 2.) The low- magnifies the image x. 3.) The high- magnifies the image eitherx or x. power objective 10 power objective 43 40

11. Using the Microscope revolving nosepiece magnification 4.) The holds the objective and can be turned to change from one to the other. 5.) The maintains the correct distance between the and . 6.) The moves the body tube and down to allow focusing of the . body tube objectives ocular lens coarse - adjustment knob image up

12. Using the Microscope fine- adjustment knob 7.) The moves the body tube to bring the image intofocus. 8.) The supports a slide. 9.) hold the slide in place for . slightly sharper stage Stage clips viewing

13. Using the Microscope diaphragm light 10.) Thecontrols that amount of coming through the stage. 11.) The light sources provides a for viewing the. 12.) Thesupports the body. light slide arm tube

14. Using the Microscope base microscope 13.) Thesupports the . 14.) Inclination joint (hinge): Allows the upper part of the microscope to be tilted back to view the specimen while seated.

15. Proper Use of the Compound Light Microscope 1.) How would you carry a microscope? • With your left hand • With your right hand • With both hands

16. Proper Use of the Compound Light Microscope 2.) How many centimeters should the microscope be from the edge of a table? • 1 cm • 5 cm • 10 cm

17. Proper Use of the Compound Light Microscope 3.) What power objective should you ALWAYS begin working with? • Low • Medium • High

18. Proper Use of the Compound Light Microscope 4.)  What must be adjusted to get the microscope into low power? • Revolving objective • Revolving nosepiece • Revolving ocular lens

19. Proper Use of the Compound Light Microscope 5.) Once you place a prepared slide over the hole in the stage, what secures the slide? • A rubber band • Your finger • Stage clips

20. Proper Use of the Compound Light Microscope 6.) If the diaphragm is moved, what gets adjusted? • The height of the stage • The amount of light • The objective lens

21. Proper Use of the Compound Light Microscope 7.) Once you are looking at the stage from eye level, you should slowly turn the: • Fine adjustment knob • Medium adjustment knob • Course adjustment knob

22. Proper Use of the Compound Light Microscope 8.) You should NEVER allow the objective to touch the slide • True • False • Sometimes it can

23. Proper Use of the Compound Light Microscope 9.) While looking through the ocular lens, you should turn the coarse adjustment knob to raise the low-power objective so that the image is: • Focused • Blurred • Not visible

24. Proper Use of the Compound Light Microscope 10.) Once the object is focused, what sharpens the image? • Fine adjustment knob • Medium adjustment knob • Coarse adjustment knob

25. Proper Use of the Compound Light Microscope 11.) When you are in high power you should always use the coarse adjustment knob? • True • False • Sometimes

26. Proper Use of the Compound Light Microscope 12.) When you are finished using the microscope, you should remove the slide and clean the ocular and objective lens with what type of paper? • Lens paper • Sand paper • Construction paper

27. Proper Use of the Compound Light Microscope 13.) When you are all finished using the microscope, you should always: • Return it to the storage area • Leave it where it is • Throw it away

28. Making a Wet Mount Slide 1.) Use sand paper to clean a glass slide and a coverslip. LENS

29. Making a Wet Mount Slide 2.) Place the specimen you wish to observe on the left of the slide. CENTER

30. Making a Wet Mount Slide 3.)  Using a medicine dropper, place 5 drops of water on the specimen. ONE

31. Making a Wet Mount Slide 4.)  Hold the coverslip at the edge of the water at a 90 degree angle to the slide. Make sure the water runs along the middle of the coverslip. 45 & EDGE

32. Making a Wet Mount Slide 5.) Lower the coverslip quickly to avoid trapping air bubbles. SLOWLY

33. Making a Wet Mount Slide 6.)  Water might evaporate from the slide as you work. Remove more water to keep the specimen fresh. ADD

34. Making a Wet Mount Slide 6a.)  Place the tip of the medicine dropper next to edge of the coverslip. Add 4 drops of water. A (ONE)

35. Making a Wet Mount Slide 6b.)  Remove excess water from the slide by using the center of a paper towel as a blotter. CORNER

36. Making a Wet Mount Slide 6c.) Lift the coverslip to add or remove water. DO NOT