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Freeze dried oven dried butanol ethanol butanol ethanol

ALPHA HELIX. B E. E. B E. BETA SHEET. Freeze dried oven dried butanol ethanol butanol ethanol. Spray dried ethanol. 0.1 mm mmmm. 0.1 mm mmmm. MAINLY HELICAL. MAINLY SHEET.

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Freeze dried oven dried butanol ethanol butanol ethanol

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  1. ALPHA HELIX B E E B E BETA SHEET Freeze dried oven dried butanol ethanol butanol ethanol Spray dried ethanol

  2. 0.1 mm mmmm 0.1 mm mmmm MAINLY HELICAL MAINLY SHEET

  3. Heat treatment during the extraction and drying process appears to cause denaturation and aggregation of kafirin, decreasing solubility. Kafirin films, where the -helical structure of the “native” protein has been retained, are better quality, smoother and more transparent, than those containing aggregated proteins with a greater -sheet content. Denatured/aggregated kafirins give less homogeneous films showing phase separation

  4. α β a b c d e FT-IR spectra of kafirin films with sorghum condensed tannin a = unmodified film, b = 5 %, c =, 10 %, d = 15 %, e = 20 % level of modification

  5. Tannic acid and sorghum condensed tannins stabilised the kafirin α-helical structure and decreased β-sheet formation Tannic acid cross-linking decreased the mobility of the kafirin polypeptide chains

  6. PREDICTED VALUE RAW DATA WITH DEUTERATED GLYCEROL CORRECTED FOR MOBILE PROTEIN

  7. GLYCEROL INCREASES THE ALPHA HELICAL CONTENT OF THE PROTEIN AND CAUSES MORE MOBILE MATERIAL TO APPEAR THAN CAN BE EXPLAINED SIMPLY BY THE AMOUNT OF PLASTICISER ADDED. Glycerol must also act as solvent

  8. heat solvent Hydrophobic interiors unwind slightly And coagulation takes place. Degree of unwinding/ coagulation is determined by the thermal history of the sample

  9. dry Tannin dry

  10. plasticiser

  11. Some absorption of glycerol plasticiser More plasticiser MOSTLY IMMOBILE RIGID CORE Remains alpha helical MOBILE Reverts to random coil or alpha helix Glycerol acts at as a solvent for the unwound region. Highly mobile region

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