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ISTA Comparative Test for Forest Tree Seeds

ISTA Comparative Test for Forest Tree Seeds. Elena Foffová Forest Research Institute Zvolen Research Station Liptovský Hrádok. Vladimír Foff Forestry Information Agency Liptovský Hrádok. CZDL02 - Výzkumní ústav lesního hospodářství a myslivosti, Výzkumná stanice Uherské Hradiště

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ISTA Comparative Test for Forest Tree Seeds

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  1. ISTA Comparative Test forForestTreeSeeds Elena Foffová Forest Research Institute Zvolen Research Station Liptovský Hrádok Vladimír Foff Forestry Information Agency Liptovský Hrádok

  2. CZDL02 - Výzkumní ústav lesního hospodářství a myslivosti, Výzkumná stanice Uherské Hradiště DEDL04 - Staatliche Landwirtschaftliche Untersuchungs- und Forschungsanstalt Augustenbers, Karlsruhe FRDL01 - Centre de Nancy, Unité Recherches Semences Forestiers, Champenoux FRDL02 - Station Nationale d´Essais de Semences, Beaucouzé GBDL02 - Official Seed Testing Station for Tree and Shrubs Seed, Alice Holt Lodge, Wrecclesham, Farnham, Surrey JPDL04 - Forestry and Forest Product Research Institute, Ibaraki NODL02 - Norwegian Forest Seed Station, Hamar RODL02 - Institutul de Cercetari si Amenajari Silvice, Laboratorul de Seminte Foresti, Bucuresti SEDL05 - Seed Laboratory, Dept. of Silviculture, The Swedish University of Agr. Science, Umea SEDL06 - Skog Forsk, The Forestry Research Institute of Sweden, Sävar SIDL01 - Kmetijski institut Slovenije, Seed Testing Labolatory, Ljubljana SKDL02 - Lesnícky výskumný ústav, Výskumná stanica Liptovský Hrádok USDL03 - NationalNational Tree Seed Laboratory, Dry Branch, Georgia ISTA Laboratories

  3. Bayerische Landesanstalt für Forstliche Saat- und Pflanzenzucht, Teisendorf Danisch Tree Improvement Station, Humlebak National Forest Genetic Resources Centre, Natural Resource Canada, Canadina Forest Servis - Atlantic, Fredericton, New Brunswick Forstliche Bundesversuchsanstalt, Abteilung für forstliches Vermehrungsgut, Wien Instytut badawczy leśnictwa, Warszawa Landesforstanstalt Eberswalde, Forstsaatgutprüfstelle Waldsieversdorf Participating Laboratories

  4. Samples and Tests

  5. Purity analysis • carried out on one whole sample by 14 laboratories • on two subsamples by 5 laboratories • Difficulties: • Results no based on sum of weights of components (3 laborotories) • Pure seed definitions No. 47 and 51: • distinguishing between winged seeds and seeds without wing (3 laboratories - no, other - big variability) • Counting mistakes, rounding

  6. Weight determination Counting the working sample - 5 laboratories Counting replicates - 14 laboratories Difficulties: One completelly wrong result, weight not calculated from replicates, coefficient of variation not given or not correct calculated, rounding

  7. Germination (conifers) Quite uniform results in category of normal seedlings at the end of the test but very inconsistent in first counting (after 7 days) Big differences in categories of the ungerminated seeds (hard, fresh, dead, empty) Difficulties: wrong counting or rounding, results not rounded

  8. Facultative tetrazolium and germination tests with beech seeds High variability in results among laboratories, among the four replicates of the test Average germination (No6 = 37%, No7 = 28%) much lower than the average viability in the TZ test (No6 = 58%, No7 = 57%) Difficulties: not correct counting of results, rounding, exceeded tolerances among replicates, not finished test

  9. Conclusions for next FTS Committee activities: Purity: to revise PSD of conifer seeds Germination:to revise the definition of „normal seedling“ TZ and germination test of dormant seeds: discuss in detail testing and evaluation procedures (substrates, evaluation of normal seedlings) Results support the FTS Committee goals: to revise the Tree and shrub seed handbook to organise this workshop with practical training

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