1 / 24

SERVIZIO BIO-PLEX CRIBI

SERVIZIO BIO-PLEX CRIBI. Bio Plex. A N A L I S I S I M U L T A N E E. Suspension Array reader. D I P R O T E I N E. Analisi qualitative e quantitative simultanee di proteine. Principio Funzionale. Principio Funzionale. Principio Funzionale. La Camera di Lettura.

renardo
Download Presentation

SERVIZIO BIO-PLEX CRIBI

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. SERVIZIOBIO-PLEXCRIBI

  2. Bio Plex A N A L I S I S I M U L T A N E E Suspension Array reader D I P R O T E I N E

  3. Analisi qualitative e quantitative simultanee di proteine

  4. Principio Funzionale

  5. Principio Funzionale

  6. Principio Funzionale

  7. La Camera di Lettura

  8. M U L T I W E L L B I O P L E X Reporter channel Classify channel

  9. Saggi per Citochine

  10. IL-2 IL-4 GmCSF TNFa Saggi per Citochine Sandwich Immunoassay - Cytokine -

  11. Kit applicativi Multiplex tested Citochine umane (27): IL-2 IL-1b IL-4 IL-5 IL-6 IL-7 IL-8 IL-12 (p70) IL-10 IL-13 IFN- IL-17 TNF- MCP-1 (MCAF) GM-CSF MIP-1b Eotaxin Rantes IL-1ra IL-9 IL-15 PDGF bFGF VEGF Nuovo kit 27-plex: 171-A11127HUMAN CYTOKINE 27-PLEX, 1X96

  12. Kit applicativi Multiplex tested Citochine di topo (23): IL-1 IL-1a MIP-1a IL-5 IL-3 Rantes IL-2 IL-6 IL-13 IL-10 IL-12 (p40) MCP1 IL-4 IL-12 (p70) Eotaxin IFN-IL-17 MIP-1 TNF- KC IL-9 GM-CSF Nuovo kit 23-plex: 171F11241MOUSE CYTOKINE 23-PLEX, 1X96

  13. Kit applicativi Multiplex tested Citochine di Ratto (9): GM-CSF IL-4 IFN- IL-6 IL-1IL-10 IL-2 TNF- Kit 9-plex: 171K11070RAT CYTOKINES 9-PLEX A PANEL,1X96

  14. Saggi per Fosfoproteine

  15. JNK IB P P P P STAT3 ERK1 Saggi per Fosfoproteine Sandwich Immunoassay – Phospho -

  16. Saggi per Fosfoproteine 15 Phosphoprotein Assays • Phospho-Erk2 • Phospho-IB- • Phospho-JNK • Phospho-p38 MAPK • Phospho-Stat3 • Phospho-ATF2 • Phospho-Erk1 • Phospho-TrkA • Phospho C-Jun • Phospho HSP 27 • Phospho p53 • Phospho NF-kβ • Phospho S6 Ribosomal P. • Phospho-Erk1/2 • Phospho-EGFR • Phospho-Akt • Phospho-GSK-3a/b • Phospho-p70 S6 • Phospho-p90 RSK • Phospho-Stat2 LISATI DI COLTURE CELLULARI LISATI DI CAMPIONI TISSUTALI

  17. Saggi per Fosfoproteine • 8 Total Target Assays • Total Erk2 • Total IB • Total p38 MAPK • Total ATF-2 • Total JNK • Total Akt • Total p90 RSK • Total c-Jun

  18. Correlazione tra Bio-Plex e W. Blot All of our cytokine and phosphoprotein detection assays are validated against ELISA and western blot to validate the results. Here we look at the correlation between the Bio-Plex phosphoprotein detection assay and western blot for Erk and Stat-3. Phospho-Erk Phospho-Stat3 Western Blot, 8 ug protein/lane Western Blot, 8 ug protein/lane Bio-Plex Assay Bio-Plex Assay 1600 1200 MFI of Bio-Plex +2SD 800 400 0 1 2 3 4 5 6

  19. Un Sistema Aperto Formati Applicabili

  20. Amine Coupling Kit

  21. Amine Coupling Kit o o R R oH C C N N H H Chimica dell’accoppiamento + = Carbodiimide reaction • Le beads vengono attivate dall’EDC • (1-ethyl-3 (3-dimethylaminopropyl) carbodiimide hydrocloride) • Reagiscono con S-NHS (N-hydroxysuccinimide) • Si formano esteri stabili che reagiscono con il gruppo NH2 della proteina per formare un legame covalente

  22. 171-406001 171-5060XX Amine Coupling Kit COOH Beads Regione XX, 1 ml Per 30 reazioni di accoppiamento Per 10 reazioni di accoppiamento Amine Coupling Kit Un coupling kit e 3 ml di beads sono sufficienti per 60 multiwell da 96 pozzetti

  23. Saggi di Interazione DNA - Proteine

  24. Interazione DNA-Proteine

More Related