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Lipase

Lipase. Prepared by Abeer Alhabash. Lipase. Lipase is an enzyme the body uses to break down fats in food so they can be absorbed in the intestines. Lipase is produced in the pancreas, mouth, and stomach. . Prepared by Abeer Alhabash. Lipase. pancreatic enzyme. Gastrointestinal.

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Lipase

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  1. Lipase Prepared by Abeer Alhabash

  2. Lipase • Lipase is an enzyme the body uses to break down fats in food so they can be absorbed in the intestines. • Lipase is produced in the pancreas, mouth, and stomach.  Prepared by Abeer Alhabash

  3. Lipase pancreatic enzyme Gastrointestinal Gastric lipase Prepared by Abeer Alhabash

  4. Gastric lipase is an acidic lipase secreted by the gastric chief cells in the fundic mucosa in the stomach. • It has pH optimum of 3-6. • Gastrointestinal lipase is secreted in an intestinal and it hydrolyze fat to fatty acid and glycerin . It has a pH optimum of 8.3 Prepared by Abeer Alhabash

  5. Pancreatic lipase is secreted from the pancreas, and is the primary lipase (enzyme) that hydrolyzes (breaks down)  fatmolecules in the human digestive system, Activated by bile salts. It has a pH optimum of 7 Prepared by Abeer Alhabash

  6. Materials Oil Alcohol Ethylic %96 Tube distill water Water bath Phenol red Na2CO3 0.5% Lipase Prepared by Abeer Alhabash

  7. Procedure A small drop of oil 4 ml ethylic alcohol concentration of 96%. 4 مل كحول إيثيلي تركيزه 96% • Take a clean tube and put a small drop of oil and add 4 ml ethylic alcohol concentration of 96%. • Add the tube in the bath water (temperature 80 -100oC) and Shake the tube to avoid vaporize of alcohol . • Add equal amount of water in tube . اضيفي 4 Add 4 ml of water مل من الماء حتي يتكون مستحلب دهني Prepared by Abeer Alhabash

  8. Add several drops of phenol red Add several drops of sodium carbonate solution 0.5% • Add several drops of phenol red and Add 2-3 drops of sodium carbonate solution 0.5%, even becomes the color of solution is pink. • Add 2 ml of lipase enzyme and put the tube in water bath at 37 ° C for a while. 2 ml of lipase enzyme 37 ° C Prepared by Abeer Alhabash

  9. Observation : Color of solution converted from pink to yellow. • Conclusion: Conversion the solution from alkaline to acidic Due to the formation of fatty acid Because the digestion of fat (oil) by enzyme lipase fatty acid + glycerin. Prepared by Abeer Alhabash

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