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MS Interpretation

MS Interpretation. Nominal M + - gives mass of ion Exact m/z (high resolution) – get stoichiometry (each atom has exact mass) Isotope Peaks C+1 = 1.1%, O+2= .2% Fragmentation – important with EI What was lost? [(M-15) + - loss of CH 3 ]. McLafferty Rearrangement.

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MS Interpretation

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  1. MS Interpretation • Nominal M+ - gives mass of ion • Exact m/z (high resolution) – get stoichiometry (each atom has exact mass) • Isotope Peaks • C+1 = 1.1%, O+2= .2% • Fragmentation – important with EI • What was lost? [(M-15)+ - loss of CH3]

  2. McLafferty Rearrangement

  3. Separations and Chromatography Chapters 26 – 28

  4. Types of Separations • Precipitation/filtration • Distillation • Extraction • Ion exchange • Chromatography • Electrophoresis

  5. Common properties exploited by separations • Solubility • Size • Polarity • Boiling point • Electrical mobility

  6. Chromatography – collection of methods that involve separation between 2 phases • Mobile phase • Stationary phase

  7. Silica Gel, Alumina, Cellulose Silca Gel SiO2 Cellulose Alumina Al2O3

  8. Elution – migration of analyte due to mobile phase. Chromatogram – Graph of signal vs elution time • More strongly retained species elute last • Analyte diluted during elution

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