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EXPERIMENTAL PATHWAYS

EXPERIMENTAL PATHWAYS. 1.What ’ s the Cyclins? 2.What ’ the relationship between cyclins and MPF?. What’s the cyclins?. 一、 MPF 卵细胞促成熟因子， matuation-promoting factor ； 细胞促分裂因子， mitosis-promoting factor ； M 期促进因子， M-phase-promoting factor ）。. 二、 Cyclins 细胞周期素.

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EXPERIMENTAL PATHWAYS

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  1. EXPERIMENTAL PATHWAYS 1.What’s the Cyclins? 2.What’ the relationship between cyclins and MPF?

  2. What’s the cyclins? • 一、 MPF 卵细胞促成熟因子，matuation-promoting factor； 细胞促分裂因子，mitosis-promoting factor； M期促进因子，M-phase-promoting factor）。

  3. 二、Cyclins 细胞周期素 yeast: Cln1,Cln2,Clin3,Clb1-Clb6 Vertabrate: A1-2、B1-3 、C、 D1- 3、E1-2、E、F、G、H等

  4. Every cyclins has a 100 amides keep sequence, which called cyclin-frame.It can interpose cyclin combine with CDK(cycling dependent kinase), different cyclins will recognize different CDK to make a cycling-complex. M-

  5. 周期蛋白激酶 CDK ( cyclin dependent kinase),

  6. Different cycling expression in different time and combine with different CDK to accommodate different CDKase activition.

  7. 注射实验表明： 1.孕酮诱导卵母细胞成熟； 2.成熟卵细胞质中，含有卵母细胞成熟 的因子，称做MPF

  8. P619 • Kinetics of Xenopus oocyte activation by progesterone and cyclinA mRNA GVBD%

  9. What’s the relationship between cycling and MPF? • cyclinA is an activator of MPF • MPF=CDC2+Cyclin B =a complex MPF

  10. Cyclin B的降解途径

  11. P34cdc2 • The yeast protein was called p34cdc2 or simply cdc2. • The first evidence of a link between cdc2 and MPF came as the result of a collaboration between yeast and amphibian research group

  12. Recall from fission yeast were shown to react specifically with the 32- and 45-kDa protein • Antibodies formed against cdc2 from fission yeast were shown to react specifically with the 32-kDa component of MPF isolated from Xenpus eggs. • This component of MPF is a homologue of the 34-kDa yeast kinase and, the machinery controlling the cell cycle in yeast and vertebrates contains evolutionarily conserved components.

  13. 2001年10月8日, • L.Hartwell、P. Nurse、T.Hunt • 因对细胞周期调控机理的研究而荣获诺贝尔生理医学奖。

  14. thank you

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