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Specimen Preparation

Specimen Preparation. Bio 498/698. Outline. Proper handling procedures Drawers Removing Labels Different Stands/Shots (Dry Mounted Specimens) Dorsal Ventral Lateral Head Cleaning Coleoptera. Proper Handling Procedures: Drawers.

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Specimen Preparation

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  1. Specimen Preparation Bio 498/698

  2. Outline • Proper handling procedures • Drawers • Removing Labels • Different Stands/Shots (Dry Mounted Specimens) • Dorsal • Ventral • Lateral • Head • Cleaning Coleoptera

  3. Proper Handling Procedures: Drawers When taking the lid off of a drawer, always slide it underneath Drawer

  4. Proper Handling Procedures: Drawers Remove the specimen(s) you are working with

  5. Proper Handling Procedures: Drawers When you are not using the drawer, always put the lid back on. (This will keep specimens clean) Make sure that lid is on all the way

  6. Proper Handling Procedures: Removing Labels • Specimen • Pinning block • Forceps Place specimen on pinning block

  7. Proper Handling Procedures: Removing Labels Squeeze forceps above the label(s) Pull down to remove labels

  8. Proper Handling Procedures: Removing Labels Always place labels back into unit tray Always keep specimen and labels together

  9. Different Stands/Shots: Dry Mounted Specimens Lateral & Head Dorsal Ventral

  10. Dorsal • Dorsal Stands • One18% grey paper • Glass slide • Wax

  11. Ventral • Ventral stands • Glass slide • Wax • Stick the top of the pin through the wax • Ventral mostly used for Lepidoptera

  12. Lateral • Lateral Stands • Stick the pin into the stand • Try to reuse previous holes to prolong stand use

  13. Head • Head stands • Same as Lateral stands • Stick pin through stand with specimen head facing up

  14. Cleaning Coleoptera: Equipment • Specimen • Pliers • 70% EtOH

  15. Cleaning Coleoptera: How to hold the pin Correct Wrong • Pliers clamped around the pin at an angle • Pliers clamped around the pin perpendicular

  16. Cleaning Coleoptera Note: when not using the EtOH, cover the container to make it last longer – it will evaporate quickly. Hold the specimen under the EtOH and swish it around

  17. Cleaning Coleoptera After cleaning, let specimen dry before imaging. EtOH doesn’t take too long to evaporate; give it a few minutes.

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