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Michael Turell, Kirti Dave, Maria Mayda , Zahra Parker, Russell Coleman, and Daniel Strickman

Evaluation of novel dipstick assays for the detection of Rift Valley fever virus (RVFV) in mosquitoes. Michael Turell, Kirti Dave, Maria Mayda , Zahra Parker, Russell Coleman, and Daniel Strickman

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Michael Turell, Kirti Dave, Maria Mayda , Zahra Parker, Russell Coleman, and Daniel Strickman

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  1. Evaluation of novel dipstick assays for the detection of Rift Valley fever virus (RVFV) in mosquitoes Michael Turell, Kirti Dave, Maria Mayda, Zahra Parker, Russell Coleman, and Daniel Strickman US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD;VecTOR Test Systems, Inc., Thousand Oaks, CA; Walter Reed Army Institute of Research, Silver Spring, MD; US Army Medical Materiel Development Activity, Fort Detrick, MD;US Department of Agriculture, Agriculture Research Service, Beltsville, MD

  2. Opinions, interpretations, conclusions, and recommendations are those of the author and are not necessarily endorsed by the U.S. Army.

  3. Research was conducted in compliance with the Animal Welfare Act and other federal statutes and regulations relating to animals and experiments involving animals and adheres to principles stated in the Guide for the Care and Use of Laboratory Animals, National Research Council, 1996. The facility where this research was conducted is fully accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care International.

  4. Overview of RVFV

  5. Many North American mosquito species are potential vectors of RVF virus Ample supply of susceptible domestic vertebrate hosts (cattle, goat, sheep) Role of deer, horses, rodents, etc. is not known POTENTIAL FOR BECOMING ESTABLISHED IN NORTH AMERICA

  6. AVAILABLE DIAGNOSTIC ASSAYS Research Laboratory based • RT-PCR • ELISA • Infectious virus assays (cell culture) Commercially available • None

  7. Positive “Control” line Negative control “Test” line RVFV-infected mosquito

  8. Schematic side view of test strip

  9. Genome Equivalents <3 <3 3.5 <3 7.9 <3 6.1 8.1 7.0 8.1

  10. Sensitivity of the dipstick based on the amount of infectious virus present

  11. Sensitivity of the dipstick based on the CT (RT-PCR) value

  12. RVF RVF SLE WN VEE JE D-1 D-2 D-3 D-4

  13. Specificity Dengue 1-4 Japanese encephalitis La Crosse St. Louis encephalitis Venezuelan equine encephalitis West Nile Yellow fever

  14. Strains of RVFV tested Strain Where isolated OS-1 Mauritania Rhod 2269/74 Zimbabwe Ar-B 1976 Central African Republic 21445 Kenya ZH501 Egypt

  15. Summary The newly developed dipsticks for RVFV are about as sensitive as the VecTest ® West Nile virus dipstick

  16. Summary The newly developed dipsticks for RVFV are about as sensitive as the VecTest ® West Nile virus dipstick The RVFV dipstick detected virus in nearly all samples containing 105 plaque-forming units of virus.

  17. Summary The newly developed dipsticks for RVFV are about as sensitive as the VecTest ® West Nile virus dipstick The RVFV dipstick detected virus in nearly all samples containing 105 plaque-forming units of virus. There was a strong correlation between the infectious virus assay titer and the CT value

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