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Introduction to DNA Sequencing Technology

Introduction to DNA Sequencing Technology. Dideoxy Sequencing (Sanger Sequencing, Chain Terminator method). Clone the fragments to be sequenced into the virus M13. Why M13? The clones that are isolated are single-stranded DNA. . Primer. ^^^. . . . . . TGATGTCGAGCGAGTCGTACGGT-----.

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Introduction to DNA Sequencing Technology