'Competent cells' presentation slideshows

Chapter8 Microbial genetics

Chapter8 Microbial genetics. 8.4 Plasmids 8.5 Genetic Conjugation, Transformation, transduction 8.6 Transposons and Insertion Sequences. 8.4 Plasmids. Circular genetic elements that reproduce autonomously and have an extra-chromosomal existence:. 1-1000 KB in size

1.28k views • 78 slides

Lecture 5. GENETICS OF PROKARYOTES

Lecture 5. GENETICS OF PROKARYOTES. 1. Basic concepts 2. The prokaryotic genome 3. The pan-genome 4. Genetic interchange and recombination 4.1. Recombination 4.2. Transformation 4.3. Conjugation 4.4. Transduction 5. Transposable elements 6. Genetic manipulation of microorganisms

569 views • 36 slides

Recombinant DNA Technology

Recombinant DNA Technology. Dr. Hui LI Office : S408 Tel: 26538722. Topic 3 Transfer of target gene into receptor cell. Procaryote cells as receptor cells. How to make competent E.coli cells?. Bacteria that are capable of uptaking the foreign DNA are called competent ;

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Site Directed Mutagenesis of Protein PurE

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Transforming E. coli with a Recombinant Plasmid

Transforming E. coli with a Recombinant Plasmid. Laboratory 5. Starting new here!!!!!. Experiments do not rely on past results. Overview . Purpose: Get the pARA-R plasmid containing the rfp gene into bacterial cells

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Analyzing Data

Analyzing Data. Transformation. The DNA molecule is hydrophilic (water-soluble) but cell membranes are made of a very hydrophobic lipid bilayer. Two means of artificial transformation commonly used in labs: electroporation and chemical transformation.

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Analyzing Data

Analyzing Data. Transformation. The DNA molecule is hydrophilic (water-soluble) but cell membranes are made of a very hydrophobic lipid bilayer. Two means of artificial transformation commonly used in labs: electroporation and chemical transformation.

561 views • 22 slides

Bacterial Transformation

Bacterial Transformation. Broad and Long Term Objective. To characterize a single clone from an Emiliania huxleyi cDNA library using sequence analysis. Research Plan. Preparation of Competent Cells and Bacterial Transformation. Growth of Transformant and Plasmid MiniPrep.

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Mutation Mountain

Mutation Mountain. CXCR4 Introduction. G protein coupled receptor (GPCR) Activated by SDF-1, a chemokine Thought to be important to two major diseases HIV- Acts as co-receptor for M Tropic Possible cure for M Tropic infections Cancer- Expressed more in breast and other cancers

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Cloning with Plasmids

Cloning with Plasmids. 1973 Genetic Engineering Invented . MARKER. Cloning: Digestion. TARGET GENE. SOURCE DNA. MARKER. Cloning: Digested Fragments. TARGET GENE. MARKER. Cloning: Ligation. DNA LIGASE. TARGET GENE. MARKER. Cloning: Recombinant Plasmid. TARGET GENE.

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CHEMOTAXIS ITS SIGNIFICANCE IN BIOLOGY AND CLINICAL SCIENCES - Elective Subject - Introduction

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iGEM Bioware

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Restriction Analysis of pDRK and pGRN Original Plasmids

Restriction Analysis of pDRK and pGRN Original Plasmids. ara C. H. i. n. d. I. I. I. amp r. P BAD. gfp. p. D. R. K. p. G. R. N. H. i. n. d. I. I. I. H. i. n. d. I. I. I. kan r.

314 views • 14 slides

Cloning a DNA segment from bacteriophage lambda

Cloning a DNA segment from bacteriophage lambda. Recombinant DNA transformed into bacterial cells Preparation of X-gal plates - by Dr. Soukup before lab Preparation of competent cells - by Dr. Soukup before lab DURING LAB: Electrophoretic analysis of restriction digests

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Recombinant DNA

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Cloning a DNA segment from bacteriophage lambda

Cloning a DNA segment from bacteriophage lambda. Recombinant DNA transformed into bacterial cells Preparation of X-gal plates - by Dr. Soukup before lab Preparation of competent cells - by Dr. Soukup before lab DURING LAB: Electrophoretic analysis of restriction digests

279 views • 13 slides

Internship Presentation

Internship Presentation. Brendon Johnson 6/11/2010. Main Project: Transformation of Arabidopsis.

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e coli advantages

Recombinant proteins are widely used in biological and biomedical research[1], and recombinant protein expression has become a commonly used tool. Nevertheless, there are still many aspects to consider before starting an expression project. For example, which expression system is chosen for obtaining the protein of interest? Should it be expressed in bacteria, in yeast, in insect cells or in mammalian cells? What is the best expression vector to be used? As to the bacterial expression system, which strain should be chosen? Is there any differences in the expression condition between the full-length protein and a protein fragment?

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Abstract

Dual Light Controlled A rabinose B iosensor.

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