'Confocal' presentation slideshows

Confocal Microscopy

5.59k views • 94 slides

CONFOCAL MICROSCOPY IN A NEW LIGHT

1.33k views • 78 slides

Triplet Blinking stable triplet state S 1 T 1 due to solvent halogens transition metals

Transition to the Dark Side. Triplet Blinking stable triplet state S 1 T 1 due to solvent halogens transition metals. Quenching. Collision with solutes Resonance energy transfer. Energy transferred to non-fluorescent solute molecule. Fluorescence Resonance Energy Transfer.

826 views • 67 slides

Brightfield microscopy

Brightfield microscopy . Generally only useful for stained biological specimens Unstained cells are virtually invisible. Oblique Illumination. Phase Contrast. http://microscopy.fsu.edu/primer/techniques/ phasegallery/chocells.html. Aberrations. Spherical aberration Most severe

822 views • 60 slides

Announcements

Announcements. Assignment due today. Pick: 1) an article, 2) a review article, You stand up there and want to convince people to listen to you. What is big idea? Why is it important? What have they done that makes it a significant advance? How have they done the significant advance.

365 views • 31 slides

Announcements

Announcements. Assignment due today. Pick: 1) an article, 2) a review article, You stand up there and want to convince people to listen to you. What is big idea? Why is it important? What have they done that makes it a significant advance? How have they done the significant advance.

314 views • 31 slides

More on smFRET and high-resolution microscopy

More on smFRET and high-resolution microscopy. You give a (brief) talk? (4 per class: 10 minute.). Apr 21 Apr 28 May 5. Last time: smFRET. Have problems with getting 100% labeling Have problems synchronizing. G-quadruplex: at least 3G, repeated 4 times.

441 views • 21 slides

5th Intensive Course on Soil Micromorphology Naples 2001

5th Intensive Course on Soil Micromorphology Naples 2001. 12th - 14th September Image Analysis. Lecture 1 Introduction. 5th Intensive Course on Soil Micromorphology - Naples 2001. Image Analysis - Lecture 1. Image Analysis (Provisional Timetable) 12th September: 13th September

310 views • 20 slides

Reduction of out of focus light

7. Fluorescence microscopy. 7.2 Confocal fluorescence microscopy. Reduction of out of focus light Excitation light excites fluorescence more or less within the whole sample Out of focus fluorescence light is not imaged sharply

187 views • 18 slides

Shallow Curves

Sometimes get wide range of GI 50 values Due to shallow/flat curves Cytostatic cell response over a wide range of doses Perhaps select a dose that gives a greater response i.e the GI 80 in order to discriminate. Shallow Curves. Strictly confidential. TTA Confocal Study.

252 views • 9 slides

Announcements

Announcements. Please pick up syllabus and index card from front desk. Fold the card lengthwise, write your name big and bold and use it as a name plate on your table. Lecture notes will be available on Blackboard before class.

223 views • 8 slides

ILLUMINATION DETECTION/ PATH IMAGING PATH. u. r. DIVIDER STRIP. q max. OBJECTIVE LENS. q min. CONFOCAL SECTION 2h THICKNESS. FOCAL (OBJECT) PLANE. w det. 100 um. 100 um. 100 um. 100 um. 100 um. 100 um. Fold Mirror. Scanning Mirror.

143 views • 1 slides