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Lab 5d SDS-PAGE

Lab 5d SDS-PAGE

Lab 5d SDS-PAGE. Electrophoresis. Separates molecules based on molecular mass and/or charge. Proteins move in the electric field. Their relative speed depends on the charge, size, and shape of the protein. What is so special about SDS?. SDS is a negatively charged detergent.

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472 views • 46 slides
Protein Apparatus

Protein Apparatus

Protein Apparatus. Protein electrophoresis. Treat with SDS before electrophoresis Makes proteins negatively charged Performs cell lysis Partially denatures proteins Then, heat at 95 degrees to fully denature proteins Also, treat with b - mercaptoethanol to break disulfide bonds.

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304 views • 20 slides
SDS-Page Part II

SDS-Page Part II

Electrophoresis and Visualization. SDS-Page Part II. Boiling Lysates. Proteins are being denatured by the combination of SDS and heat. Resultant proteins take on a rod-like shape and a uniform negative charge-to-mass ratio proportional to their molecular weight. Electrophoresis.

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354 views • 15 slides
Supplemental Figure 1

Supplemental Figure 1

Supplemental Figure 1. Chlorophyll fluorescence (rel). 1 min. pgr5 pgr5 + NEM (0.5mM).

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99 views • 5 slides
kDa

kDa

Supplementary Figure 1. A). Fr:. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. kDa. 49. 37. 26. 19. 15. 6. B). Fr:. kDa. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 182.

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156 views • 1 slides
JUP

JUP

A. Soluble. Insoluble. Soluble. Insoluble. WT+ Mut. WT+ Mut. WT+ Mut. WT+ Mut. WT. Mut. WT. Mut. WT. Mut. WT. Mut. JUP. DSC2/3. DSG2. DSP. Coomassie Blue. Coomassie Blue. B. WT. Mut. WT. Mut. Cyt. NE. Cyt. NE. Cyt. NE. Cyt. NE. Sol. Insol.

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156 views • 1 slides

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