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Large scale sequencing - PowerPoint PPT Presentation

Next Generation Sequencing Data Analysis

Next Generation Sequencing Data Analysis. Nadia Pisanti, University of Pisa. Why sequencing?. The knowledge of DNA and RNA sequences has become a crucial tool for: Basic research in biology, pharmacology and medicine.

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973 views • 31 slides

Fig. 20-11

Northern Blot (to detect CD4 mRNA). TECHNIQUE. Heavy weight. I II III. RNA. Nitrocellulose membrane (blot). Gel. Sponge. T cells. B cells. macrophage. Paper towels. Fig. 20-11. Alkaline solution. 2. 1. 3. Preparation of restriction fragments. DNA transfer (blotting).

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378 views • 23 slides

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Large scale sequencing - PowerPoint PPT Presentation

Next Generation Sequencing Data Analysis

Fig. 20-11