IMPACT OF AO BIOTICS EQE ON EGG PRODUCTION AND SURVIVABILITY OF LAYING HENS IN FRANCE

1. IMPACT OF AO-BIOTICS® EQE ON EGG PRODUCTION AND SURVIVABILITY OF LAYING HENS IN FRANCE C. Ocasio-Vega AO-Biotics EQE had a positive impact on egg production and survivability in this on-farm validational trial. DOSE USED 50 g/ton SUMMARY Supplementation of AO-Biotics EQE, a postbiotic derived from Aspergillus oryzae, improved flock lifespane and productivity of laying hens evaluated for 9 weeks, beginning at 31 weeks of age. VALUE The inclusion of AO-Biotics EQE in the nutritional program for laying hens contributes to improving egg production and extending the productive lifespan of the flock. PROTOCOL Location • Commercial laying hen operation in France. Duration • The impact of EQE was evaluated for 9 weeks using a 3-phase protocol. • From 22 to 30 weeks of age, animals were fed a basal, unsupplemented diet. • From 31 to 39 weeks of age, animals were fed the same diet as above with 50 g/ton EQE. • From 40 to 45 weeks of age, animals were returned to the basal, unsupplemented diet.

2. PROTOCOL (CONTINUED) Animals • Approximately 100,000 Lohman LSL (white) laying hens housed in a single barn. Trial Design • An “OFF-ON-OFF” design was used. • Hens were evaulated without supplementation, with supplementation, and then without supplementation once again. Treatments • Basal diet with no postbiotic supplementation. • Treatment diet with postbiotic added at a dose of 50 g AO-Biotics EQE per ton of feed. Data Collection • Laying hen production traits 1. Egg rate 2. Feed intake 3. FCR 4. Mortality • Egg quality traits 1. Egg weight and mass 2. Total number of eggs produced RESULTS Compared to both OFF phases, AO-Biotics EQE improved hen performance (Figure 1, Tables 1-2). • Improved feed intake by 4.4% • Improved egg mass by 4.4% • Improved FCR by 7.6% • Improved egg production by 3.1% • Increased number of eggs sold by 54,000

3. RESULTS (CONTINUED) A) Feed intake, g/d B) Egg mass, g C) FCR, g:g 70.00 3.00 150.00 65.00 2.50 130.00 Feed intake, g/d 60.00 Egg mass, g 2.00 55.00 FCR, g:g 110.00 50.00 1.50 90.00 45.00 1.00 40.00 70.00 0.50 35.00 50.00 30.00 0.00 22-24 25-27 27-30 31-33 Weeks of age 34-36 37-39 40-42 43-45 22-24 25-27 27-30 31-33 Weeks of age 34-36 37-39 40-42 43-45 22-24 25-27 27-30 31-33 Weeks of age 34-36 37-39 40-42 43-45 D) Egg production, % E) Egg weight, g F) Eggs × hen × week, n 100 70.00 7.50 Eggs × hen × week, n Egg production, % 95 60.00 7.00 Egg weight, g 90 50.00 6.50 85 40.00 6.00 80 30.00 5.50 75 20.00 5.00 22-24 25-27 27-30 31-33 34-36 37-39 40-42 43-45 22-24 25-27 27-30 31-33 Weeks of age 34-36 37-39 40-42 43-45 22-24 25-27 27-30 31-33 34-36 37-39 40-42 43-45 G) Total eggs sold/phase, n 2200000 Total eggs sold/phase, n 2100000 2000000 1900000 1800000 1700000 1600000 22-24 25-27 27-30 31-33 Weeks of age 34-36 37-39 40-42 43-45 Figure 1. Impact of AO-Biotics EQE on laying hen performance (OFF phases shown in blue, ON phase shown in red).

4. RESULTS (CONTINUED) Table 1. Performance and egg quality of hens fed a diet supplemented with AO-Biotics EQE at 50 g/ton of feed from 31 to 39 weeks of age1.

5. RESULTS (CONTINUED) Table 2. Linear regression equations for the productive performance and egg quality of hens fed a diet supplemented with AO-Biotics EQE from 31 to 39 weeks of age. During the ON phase when EQE was supplemented, variability in egg production was reduced by 46% and variability in egg mass was reduced by 48% when compared to the OFF phases. As a note, normal physiological changes occuring during the laying cycle of the various phases may contribute to these results. BIOZYME INCORPORATED 6010 Stockyards Expy | St. Joseph, MO 64504 USA Tel: 816-238-3326 | Fax: 816-238-7549 support@biozymeinc.com | www.biozymeinc.com