Impact of AO-Biotics EQE on Egg Production and Survivability of Laying Hens in Mexico from 14 to 45 Weeks of Age

1. IMPACT OF AO-BIOTICS® EQE ON EGG PRODUCTION AND SURVIVABILITY OF LAYING HENS IN MEXICO FROM 14 TO 45 WEEKS OF AGE C. Ocasio-Vega AO-Biotics EQE had a positive impact on egg production and survivability in this on-farm validational trial. DOSE USED 50 g/ton SUMMARY Supplementation of AO-Biotics EQE, a postbiotic derived from Aspergillus oryzae, improved egg production, and egg mass. The addition of EQE in the diet also improved the survival rate of hens in the trial. VALUE The inclusion of AO-Biotics EQE in the nutritional program for laying hens contributes to improving egg production while extending the productive lifespan of the flock. PROTOCOL Location • Commercial laying hen operation in Mexico. Duration • The impact of EQE was evaluated for 31 weeks from 14 to 45 weeks of age. Animals • Approximately 80,000 Bovans and Hi-Line laying hens evenly distributed in four barns.

2. PROTOCOL (CONTINUED) Trial Design • Control hens were provided a standard diet. • The other recieved the control diet with 50 g/ton of AO-Biotics EQE added to the feed. Treatments • Control with standard diet - Bovans and Hi-Line fed separately. • Treatment group with EQE postbiotic added at a dose of 50 g per ton of feed - Bovans and Hy-Line fed separately. Data Collection • Laying hen production traits 1. Egg rate 2. Egg weight and mass 3. Feed intake 4. FCR 5. Mortality 6. Total eggs produced RESULTS AO-Biotics EQE increased egg production and egg quality parameters (Table 1, Figures 1 - 2), including: • Egg mass by 5.76%. • Egg production by 12.1%. • Overall egg production, resulting in 0.72 more eggs laid.

3. RESULTS (CONTINUED) Table 1. Mean performance and egg quality of hens fed control or EQE-supplemented diets from 14 to 45 weeks of age1. Figure 1. Impact of AO-Biotics EQE on hen productivity from 14 to 45 weeks of age (Bov = Bovans, Hi-L = Hi-Line).

4. RESULTS (CONTINUED) Figure 2. Impact of AO-Biotics EQE on number of eggs laid and number of eggs sold from 14 to 45 weeks of age (Bov = Bovans, Hi-L = Hy-LIne). The survivability of layers was also improved when EQE was added to the diet. Compared to the control group, the treatment group survivability was increased by 32.7% (Figure 3). Figure 3. Impact of AO-Biotics EQE on accumulated mortality from 14 to 45 weeks of age (Bov = Bovans, Hi-L = Hi-Line). BIOZYME INCORPORATED 6010 Stockyards Expy | St. Joseph, MO 64504 USA Tel: 816-238-3326 | Fax: 816-238-7549 support@biozymeinc.com | www.biozymeinc.com