Impact of AO-Biotics EQE on Egg Production and Survivability of Laying Hens in Mexico from 15 to 51 Weeks of Age

1. IMPACT OF AO-BIOTICS® EQE ON EGG PRODUCTION AND SURVIVABILITY OF LAYING HENS IN MEXICO FROM 15 TO 51 WEEKS OF AGE C. Ocasio-Vega AO-Biotics EQE had a positive impact on egg production and survivability in this on-farm validational trial. DOSE USED 50 g/ton SUMMARY Supplementation of AO-Biotics EQE, a postbiotic derived from Aspergillus oryzae, improved hen productivity, egg mass, and egg weight. VALUE The inclusion of AO-Biotics EQE in the nutritional program for laying hens contributes to improving egg production through increased weight and mass. PROTOCOL Location • Commercial laying hen operation in Mexico. Duration • The impact of EQE was evaluated for 36 weeks from 15 to 51 weeks of age. Animals • Approximately 70,000 Hi-Line laying hens evenly distributed in two barns.

2. PROTOCOL (CONTINUED) Trial Design • Control hens were provided a standard diet. • The other recieved the control diet with 50 g/ton of AO-Biotics EQE added to the feed. Treatments • Control with standard diet. • Treatment group with EQE postbiotic added at a dose of 50 g per ton of feed. Data Collection • Laying hen production traits 1. Egg rate 2. Egg weight and mass 3. Feed intake 4. FCR 5. Mortality • Egg quality traits 1. Percentage of discarded eggs 2. Total number of eggs produced 3. Total number of eggs by grade RESULTS AO-Biotics EQE increased egg production and egg quality parameters (Table 1, Figure 1). Layers fed EQE produced eggs with 1% more mass than controls.

3. RESULTS (CONTINUED) Table 1. Mean performance and egg quality of hens fed control or EQE-supplemented diets from 15 to 51 weeks of age1.

4. RESULTS (CONTINUED) Figure 1. Impact of AO-Biotics EQE on hen productivity from 15 to 51 weeks of age. BIOZYME INCORPORATED 6010 Stockyards Expy | St. Joseph, MO 64504 USA Tel: 816-238-3326 | Fax: 816-238-7549 support@biozymeinc.com | www.biozymeinc.com