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Purified Manganese oxide E-training #7| Virtual | 17/05/21
Animine product portfolio: Purified manganese oxide Potentiated zinc oxide Monovalent copper oxide Metal oxide ….enriched with the new product: ManGrin® A CONSISTENT OFFER: Unique color Unique on the market
ManGrin®unique manufacturing process High Grade Mn ore Secondary purification Primary purification Impurities Contaminants ManGrin®is the only purified MnO source : -> High Mn concentration -> Low levels of contaminants
Metal concentrations of feed grade Mn sources Manganese sources Minimum Mn concentration* ManGrin® Manganese (II) oxide Dimanganese chloride trihydroxyde 75 % 60 % 44 % Manganous sulphate monohydrate 31 % Manganous chloride tetra hydrate Manganese chelate 27 % 8 - 16 % *EU Regulation Mn concentration on different chelated sources ManGrin® has the highest Mn concentration in the market
Technological properties – Manganese sources are variable Manganese sulfate Manganese chelate Standard manganese oxides ManGrin®is a highly free flowing powder for optimal productivity and homogeneous mixing.
Particle size distribution Dust /airborne ManGrin® MnO-1 MnO-2 MnO-3 ManGrin®guarantees dust free powder with high flowability
Neurotoxicity of manganese ✓ Manganese is considered as a neurotoxic ✓ Inhalation exposure to manganese is associated to the development of adverse neurological, reproductive, or respiratory effects ✓ Manganese toxicity leads to similar neuropathology as Parkinson’s disease Manganese is a higher concern for workers’ safety than other essential metals
Manganese and occupational health ✓ Premix and feed manufacturers must protect their workers from inhalation risks of critical feed additives ✓ Inhalation levels are measured by Occupational Exposure Limits (OELs) which are country-specific ✓ In the European Union, recent EU Directive 2017/164 defined stricter OELs for manganese exposure ManGrin®is a dust free product which helps premix and feed companies to secure their working place
Contaminants: heavy metals and dioxins Other Mn sources EU directive 2002/32 MnO EU directive 2002/32 Contaminants ManGrin® Arsenic 30 - 50 100 10 Cadmium 10 - 30 30 5 Lead 100 200 50 Dioxins (ng) 1.5 1.5 1.5 Maximum content (mg/kg) ManGrin® has high purity with very low levels of contaminants
Example: contribution to arsenic content in feed MnSO4 AA-chelate Met-chelate MHA-chelate Gly-chelate Standard MnO TBMC ManGrin® 0 50 100 150 200 % 250 300 350 400 Lower contaminants X lower inclusion level = very low supply of heavy metals
Bioavailability of manganese sources : high variability Mn content, % 31,4 64,2 45,6 58,1 RBV, kidney, % to sulfate 100 68 52 53 MnSO4 MnO – Feed Grade 1 MnO – Feed Grade 2 MnO – Feed Grade 3 From Wong-Valle et al, 1989 Mn absorption from MnO influenced by MnO purity Particle size MnO2can be up to 8% if not controlled Other metals : Iron (2-8%) Mean particle size of standard MnO can vary from 20 to 130 µm
ManGrin®is a stable source of MnO Metal oxides are not soluble at neutral pH: Less interactions in the feed Less interactions in the premix Less interaction in the upper GIT (including the rumen)
Bioavailability study 280 broilers (Ross 308) 7 treatments: 5 birds/treatment - 8 replicates each Day-old birds were set in one of treatments for 35 days Zootest Treatments Negative control 30 ppm Native Mn Basal diet no Mn supplementation At animal requirement MnSO4 MnO Basal + 30 ppm Mn supplemented from or or ManGrin® (EU Standard) (EU Standard) Above animal requirement MnO MnSO4 (EU Standard) Basal + 60 ppm Mn supplemented from or or ManGrin® (EU Standard) Measurements Feed analysis Mn deposition liver and bone at 35 days
Bioavailability results Bone Mn 9 a a a 8 7 b 6 Above animal requirement, bone Mn concentration is not a relevant biomarker of Mn status Mn (mg/kg) 5 4 3 2 1 0 P < 0,05 NC NC MnSO4 MnSO4 MnO A MnO ManGrin ManGrin® Based on bone Mn, ManGrin®is as bioavailable as MnSO4
Bioavailability results Liver Mn 3 a 2.8 ab 2.6 ab Mn (mg/kg) 2.4 b 2.2 2 1.8 1.6 NC MnO A MnO ManGrin ManGrin® P < 0,05 MnSO4 MnSO4 NC Based on liver Mn, ManGrin®showed the highest bioavailability
Absorption and storage of Mn in the body Liver is a more interesting biomarker to assess Mn bioavailability Bone mineralization Mn Absorption through the gut mucosa = Mn bioaccessibility Mn storage in liver = Mn bioavailability Other functions (enzymes, collagen formation, reproduction…) Highly labile Mn from the liver is available for mobilization when needed
Bioavailability results Relative Biological Value (RBV) 3.2 ManGrin® 3.0 133% Mn content in liver (mg) 2.8 MnSO4 100% 2.6 MnO 79% 2.4 2.2 2.0 2 3 4 5 6 7 8 9 10 11 Mn daily intake (g) RBV of ManGrin®is 133% when compared to MnSO4
ManGrin®positioning for laying hen ✓ Improving eggshell quality in laying hen and breeders ✓ Extended lay period in laying hens increases downgraded egg quality Laying hens Manganese might be a limiting factor for eggshell quality
ManGrin®positioning for laying hens ✓ Fortify laying hen diet with 50 to 100 ppm ManGrin® ✓ Depending on ✓ Eggshell quality issues ✓ Current Mn supply Laying hens ✓ Replace current Mn source with ManGrin® ✓ Secure supply Mn to the hen ✓ Avoid interaction ✓ Especially when : low native Mn and standard MnO as the sole Mn source Use ManGrin to secure Mn supply and increased requirement
Animix Nutritional Poultry ✓ Combine Mn, Zn and Cu supply with Animix Nutritional Poultry ✓ HiZox : increased gut integrity and support nutrient absorption ✓ CoRouge : Cu role in the formation of lysine derived cross-links of the shell ✓ Non water soluble, reduced interactions, high purity and constant products Convenient all in one solution Supplementation or replacement, including chelates
ManGrin®positioning in sows ✓ Gilts and sows are affected by lameness ✓ Trace elements are involved in cartilage & bone development as well as hoof quality ✓ Lameness is an important cause for culling ✓ Higher sow productivity and larger litter have increased Mn requirements ✓ Failure to reproduce is the second cause for culling ✓ Supplement gilt and sow diet with 50 ppm ManGrin ✓ Support gilt development and sow longevity
Animix Sow pack ✓ Zn for sows ✓ Earlier evidence of Zn positive impact on sow reproduction (Hill, 1983) with more recent confirmation (2020) ✓ Potential Zn depletion during gestation and lactation ✓ HiZox effect on intestinal microbiota ✓ Reduce fecal E. coli excretion ➔ reduce piglet contamination ✓ HiZox effect on sow performance ✓ Born alive and piglet BW at 24 h (TS 87)
Animix Sow pack ✓ Combine Mn, Zn and Cu supply with Animix Sow Pack ✓ All Animine products benefits in a pack Adjust current supply of mineral premix to optimize levels Replacement of chelates
Market approach ✓ Mn is not in the spotlight and Mn source with no support ✓ Our Mn focus is a chance to catch up interest of customer ✓ No information on standard MnO and MnSO4 : Quality ? ✓ Animine brings technical and scientific support on Mn and on ManGrin® ✓ What is true for our product is not true for others ✓ A chance for customer differentiation ✓ Something new, something different from chelates ✓ ManGrin® alone ✓ 1. On top single supplementation for testing ✓ 2. If positive, premix integration or Animix ✓ Animix ✓ Concept integration, premix or feed diffenciation (High 6) ✓ More competitive than single products ✓ 1 customer / country in 2021
Conclusions ✓ Mn is an essential nutrient for animal performance and welfare, with positive effects on the quality/safety of edible animal products ✓ Mn requirements maybe higher than current recommendations ✓ Mn sources of variable and unknown quality and availability ✓ Specific physiological functions can be improved by more bioavailable manganese sources ✓ ManGrin®is a safe source of Mn for the animals, for the consumers, for the environment, but also for colleagues ManGrin® is the most preferred source of Mn in animal nutrition
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