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pcDNA5 PGK pcDNA5 PGK

MoL #33472. shPGK shPGK + dox 0.05ng/ml shPGK + dox 5ng/ml shPGK shPGK + dox 0.05ng/ml shPGK + dox 5ng/ml. Sh-control Sh-control + dox 5ng/ml Sh-control Sh-control + dox 5ng/ml. shHIF-1 α shHIF-1 α + dox 5ng/ml shHIF-1 α shHIF-1 α + dox 5ng/ml. Supplemental Fig.5. pcDNA5 PGK

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pcDNA5 PGK pcDNA5 PGK

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  1. MoL #33472 shPGK shPGK + dox 0.05ng/ml shPGK + dox 5ng/ml shPGK shPGK + dox 0.05ng/ml shPGK + dox 5ng/ml Sh-control Sh-control + dox 5ng/ml Sh-control Sh-control + dox 5ng/ml shHIF-1α shHIF-1α + dox 5ng/ml shHIF-1α shHIF-1α + dox 5ng/ml Supplemental Fig.5 pcDNA5 PGK pcDNA5 PGK Hypoxia - - + + - - + + - - + + - - - + + + HIF-1α PGK APE-1 Supplemental Figure 5. Effect of over-expression of PGK on the levels of L-OddCTP and dFdCTP and their DNA incorporation under normoxic and hypoxic conditions in RKO cells (First column). Effect of induction shHIF-1 using doxcycline on the HIF-1 and PGK expression and the level of L-OddC, dFdCTP and their DNA incorporation under normoxic and hypoxic conditions in RKO cells (Third column). Effect of the induction of shPGK using doxcycline on PGK expression and the levels of L-OddC, dFdCTP and their DNA incorporation under normoxic and hypoxic conditions RKO cells (Fourth column).

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