Materials synthesized or used in the study CPT-LA 25 NCs Experimental procedures:

1. Experiment No: 2 complete/incomplete Exp. Name: Formation and Characterization CPT-LA25 NCs Location: YQ-2-7 This page has been reviewed and signed by _________: on: ________ Goal or motivation of this exp: to study controlled formation of CPT-PLA Nanocaonjugates and how their particle size is related to their concentration Reaction Scheme, Figure or Tables All Font size above this line should be minimum 14 and preferred to be 16 or larger). Figure caption: (a) Formation and Characterization of CPT-PLA NCs (b) CPT-LA25 NC size versus the concentration of CPT-LA25 with the use DMF as solvent for nanoprecipitation. The volume ratio of organic solvent to water was fixed at 1/20. Materials synthesized or used in the study CPT-LA25 NCs Experimental procedures: A DMF solution containing the CPT-LA25 conjugates (100L, 10mg/mL, 5mg/mL, 2mg/mL) was added dropwise to the DI water (2mL), respectively. The resulting CPT-LA25 NCs were characterized by DLS for particle size. Results: The line indicates the linear correlation of the NC size with the concentration of CPT-LA25. Discussion: When the polymer concentrations were varied during the NPP of CPT-LA25 at a fixed solvent :water ratio, we observed a linear relationship of NC size with CPT-LA25 concentration. The size of NC increased from 49.7nm to 72.8 nm as the polymer concentration in DMF increased from 2mg/mL to 10mg/mL. In DMF/water NPP system, the polydispersities of NCs at all concentrations remained very low. Because of the linear correlation of NC size with the concentration of CPT-LA25 conjugate during NPP, NCs with any desirable sizes in a certain rang can be obtained simply by adjusting precipitation concentration. Qian Yin 2

2. Experiment No: 2 complete/incomplete Exp. Name: Formation and Characterization CPT-LA25 NCs Location: YQ-2-7 This page has been reviewed and signed by _________: on: ________ Goal or motivation of this exp: to study controlled formation of CPT-PLA Nanocaonjugates and how their particle size is related to their concentration Continued: Table 1. CPT-LA25Nanoconjugates Cover as much space as possible from left to right; the width of figure or table prefer 6 inch or larger Qian Yin 2