1 / 2

Online Resource 3

Online Resource 3. S5. S4. S6. S7. Online Resource 3

strom
Download Presentation

Online Resource 3

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Online Resource 3 S5 S4 S6 S7 Online Resource 3 S5/S6 Sensitivity to chloroquine and bafilomycin A1ZR-75-1cells were exposed to various doses of Bafilomycin A1 and Chloroquine and toxicity was assessed by the MTT assay 72 hours following initial drug treatment. Adriamycin was used as a positive control (S5). Cells were exposed to 100nM 1,25D3, 200nM Bafilomycin A1 or 5µM Chloroquiune and viable cell number was assessed by trypan blue exclusion at days 3 and 6 post treatment (S6) S7 Quantification of AVOs.Percentage of cells with positive AVO staining was monitored using flow cytometry at 72 hours post treatment. Values shown are from a representative experiment with triplicate samples for each condition #p<0.05 compared to IR. ##p<0.05 compared to 1,25D3+IR

  2. Online Resource 3 S8 S9 S8/S9 Effects of pharmacologic autophagy inhibition on cell viability in IR and 1,25D3+IR treated cells ZR-75-1 cells were exposed to CQ, IR, 1,25D3+IR with and without CQ for 72 hours andcell viability was monitored by propidium iodide staining. Staining was quantified using flow cytometry. Positive staining is indicated by cells gated in the M1 region (S8). ZR-75-1 cells were exposed to CQ, IR+ CQ or 1,25D3+IR +CQ for 72 hours andapoptosis was monitored by annexin v/propidium iodide staining by flow cytometry (S9).

More Related