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Man-made vs. Herbal Remedies

Man-made vs. Herbal Remedies. By: Gillian Chandler. Question:. Are herbal remedies more effective in killing bacteria than man-made medicines? I chose to do this experiment because. Research:. Peppermint extract is commonly used to treat an upset stomach or aid digestion

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Man-made vs. Herbal Remedies

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  1. Man-made vs. Herbal Remedies By: Gillian Chandler

  2. Question: Are herbal remedies more effective in killing bacteria than man-made medicines? I chose to do this experiment because...

  3. Research: • Peppermint extract is commonly used to treat an upset stomach or aid digestion         - It also has been proven to be antibacterial • Dandelion root can help digestion and be used as a diuretic • Penicillin is used to kill bacteria in a wide range of Gram-positive bacterias         -Many bacterias have built up resistance to penicillin  • Erythromycin is an antibiotic used to treat certain bacterial infections

  4. Research (2): • Escherichia coli (E. Coli)  • Gram negative • bacterial infection  • causes food poisoning • Bacillus cereus (B. Cereus) •  Gram positive •  bacterial infection  • causes food borne illness

  5. Research (3): "Gram" refer how a bacteria reacts to an initial strain Gram positive • has many thick layers of peptidoglycan (sugar protein shell) • Effect on antibiotics Gram negative • cell wall has only contain a few layers of peptidoglycan • outer membrane that covers wall • inner membrane • Effect on antibiotics

  6. Hypothesis: If both man-made and herbal remedies are used against bacteria, then the man-made ones will be more effective.

  7. Materials: • 1 molar dandelion root solution • 1 molar peppermint solution • Distilled Water • E.Coli  • Bacillus cereus • Penicillin • Erythromycin • 20 Petri dishes • tryptic soy agar • Bunsen burner • Tweezers • Inoculation loop

  8. Procedure: • Place the gram negative bacteria in ten of the petri dishes, and the gram positive bacteria in the other ten petri dishes • Create the controls of water and blank discs, and place two in each of the four control dishes (2 b. cereus 2 e. coli)M • Make two dishes with four discs of dandelion root extract with E. Coli, and likewise with B. Cereus • Make two dishes with four discs of peppermint extract with E. Coli, and likewise with B. Cereus • Make two dishes with four discs of penicillin with E. Coli and likewise with B. Cereus • Make two dishes with four discs of erythromycin with E. Coli and likewise with B. Cereus • Allow the bacterias to incubate • Measure the zone of inhibition

  9. Data: Bacillus Cereus Data

  10. Data (2): Escherichia Coli Data

  11. Data Standard Deviation- statistical test of how legitimate your data is

  12. Discussion My hypothesis: If man-made and herbal remedies are used against bacteria, then the man-made ones will be more effective. My hypothesis was partially supported. The Bacillus Cereus results proved my hypothesis true. • the erythromycin had a greater zone of inhibition than the natural remedies The Escherichia Coli results proved my hypothesis false. • the peppermint had a greater zone of inhibition than the man-made remedies. This is because...

  13. Discussion (2): Improvements:     -use different types of herbal remedies     -use stronger antibiotics

  14. Thank You Blaze. “Herbal Remedy Chart.” Gardens Ablaze. N.p., 8 Oct. 2010. Web. 11 Dec. 2011. <http://www.gardensablaze.com/‌HerbRemedies.htm>. Caine, Philip. “2008 Project Summary.” California State Science Fair. USC, n.d. Web. 18 Sept. 2011. <http://www.usc.edu/‌CSSF/‌History/‌2008/‌Projects/‌J1805.pdf>. Carter, J. Stein. “Bacteria.” Biology. CLC, 2 Nov. 2004. Web. 18 Sept. 2011. <http://biology.clc.uc.edu/‌courses/‌bio106/‌bacteria.htm>. “E.Coli Infection- Home Treatment.” WebMD. WebMD, n.d. Web. 18 Sept. 2011. <http://www.webmd.com/‌a-to-z-guides/‌e-coli-infection-home-treatment>. Healthwise Staff. “E. Coli Infection- Treatment Overview.” Everyday Health. N.p., 14 June 2010. Web. 18 Sept. 2011. <http://www.everydayhealth.com/‌health-center/‌e-coli-infection-treatment-overview.aspx>. Rubin, Julian T. “E. Coli.” Juliant Rubin. N.p., n.d. Web. 18 Sept. 2011. <http://www.juliantrubin.com/‌topicprojects/‌ecoliprojects.html>. Sace, J. “Gram Positive vs. Gram Negative Bacterial Cell Walls.” Bright Hub. Bright Hub, 1 Apr. 2011. Web. 19 Dec. 2011. <http://www.brighthub.com/‌science/‌medical/‌articles/‌20274.aspx>. Todar, Kenneth. “Bacillus Cereus.” Textbook of Bacteriology. Kenneth Todar, Fall 2008. Web. 14 Dec. 2011. <http://textbookofbacteriology.net/‌B.cereus.html>.

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