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Figure S1

non. -. Tg/STg. +. Dox. 200. DTg. +. Dox. 180. 160. 140. 120. Relative CD4 MFI (%). 100. 80. 60. 40. 20. 0. Thymus. pLN. non. -. Tg/STg. +. Dox. DTg. +. Dox. 20. p. =0.002. p. =0.001. p. =0.004. T cells. 15. p. =0.0005. +. 10. % of CD4. 5. 0. hi. hi.

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Figure S1

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  1. non - Tg/STg + Dox 200 DTg + Dox 180 160 140 120 Relative CD4 MFI (%) 100 80 60 40 20 0 Thymus pLN non - Tg/STg + Dox DTg + Dox 20 p =0.002 p =0.001 p =0.004 T cells 15 p =0.0005 + 10 % of CD4 5 0 hi hi hi lo CD69 CD25 CD44 CD45RB Figure S1 F176042 Females Thymus Thymus A B non non - - Tg/STg Tg/STg + + Dox Dox p p =0.2 =0.2 DTg DTg + + Dox Dox 20 20 non non - - Tg/STg Tg/STg + + Dox Dox p p =0.1 =0.1 18 18 200 200 ) 7 7 DTg DTg + + Dox Dox 16 16 180 180 14 14 160 160 12 12 140 140 10 10 120 120 8 8 Relative CD4 MFI (%) Relative CD4 MFI (%) Cell numbers (X10 100 100 6 6 80 80 4 4 p p =0.006 =0.006 60 60 1.5 1.5 40 40 p p =0.02 =0.02 1.0 1.0 20 20 0.5 0.5 0 0 0 0 Thymus Thymus pLN pLN Total Total CD4 SP CD4 SP CD8 SP CD8 SP DP DP D pLN pLN C non non - - Tg/STg Tg/STg + + Dox Dox DTg DTg + + Dox Dox 22 22 20 20 non non - - Tg/STg Tg/STg + + Dox Dox p p =0.002 =0.002 p p =0.001 =0.001 20 20 p p =0.004 =0.004 DTg DTg + + Dox Dox 18 18 T cells T cells 15 15 16 16 p p =0.0005 =0.0005 14 14 p p =0.009 =0.009 + + 12 12 10 10 Cell numbers (X106) 10 10 8 8 % of CD4 % of CD4 6 6 5 5 4 4 2 2 0 0 0 0 Total Total CD4 CD4 CD8 CD8 B220 B220 hi hi hi hi hi hi lo lo CD69 CD69 CD25 CD25 CD44 CD44 CD45RB CD45RB F176043 Females E F H G I Rahim et al. 

  2. Thymus (Female F148571) - Dox + Dox 6.3±2 88±5.5 6±2.2 87.2±4.3 CD4 2.2±1.8 2.3±1 CD8 Figure S2

  3. Figure S3 Rahim et al. 

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