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Varian 900-LC Series i HPLC

Varian 900-LC Series i HPLC. What is the i HPLC?. 2 models 920-LC and 940-LC Compact, stylish, automated analytical and semi prep HPLC Pre-plumbed and factory tested for the highest confidence Easy to order, install and use Single Ethernet cable connection

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Varian 900-LC Series i HPLC

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  1. Varian 900-LC Series iHPLC

  2. What is the iHPLC? • 2 models 920-LC and 940-LC • Compact, stylish, automated analytical and semi prep HPLC • Pre-plumbed and factory tested for the highest confidence • Easy to order, install and use • Single Ethernet cable connection • Focused on improved productivity through automation • Updated premium performance hardware • Autosampler, Fraction Collector and Scale up Module for semi-prep • Quaternary pump, precision Column Heater,RI, Fluor, ELSD and autosampler for analytical • Updated Integrated Instrument Software Interface • Eliminate repetition, modernise UI • Consistent • Easy to use with added functionality • Adds to modular range

  3. Varian 920-LC Integrated Analytical HPLC

  4. Varian 920-LC What Is It? • Compact, stylish automated analytical HPLC • Pre-plumbed and factory tested for the highest confidence • Single cable communications • Virtually pulse-free dual piston pump • Does not require a pulse damper • State-of-the-art low pressure quaternary gradient module with built-in degasser • Autosampler with cooling option • Synchronised injection for outstanding retention time reproducibility • Compatible with 200 x 1.6 ml HPLC vials or 3 microplates

  5. Varian 920-LC What Is It? • Either dual l UV-Vis or PDA detector • Optional second detector - Fluorescence or RI • High precision (<0.15oC) dual column heater available • Low delay solvent pre-heater for improved heat transfer • Optional column switching and electric injection valve • NEW improved instrument interface on GalaxieTM software • External CVM, ELSD or 356-LC RI can be plugged directly into the back of the unit.

  6. Varian 920-LC Silver Bullets • Premium analytical pump performance and reliability • Superb Fast LC performance optimised for sub 3 um columns • Improved throughput without compromising robustness • High sensitivity/top line performance detection systems across UV/PDA/RI/Fluor and ELSD – unparalleled strength • Unbeatable Injection performance and carryover statistics with 2008 loopless technology autosampler • Full system level computer controlled AIQ (validation) • Updated driver for enhanced user experience

  7. 920-LC from Conventional to Fast HPLC 25cm x 4.6mm Pursuit XRS 5um 1.0ml/min 25cm x 4.6mm Pursuit XRS 5um 2.2ml/min 15cm x 4.6mm Pursuit XRS 5um 1.0ml/min 15cm x 4.6mm Pursuit XRS 5um 3.75ml/min 10cm x 2.0mm Pursuit XRS 2.8um Ultra 0.8ml/min 5cm x 2.0mm Pursuit XRS 2.8um Ultra 0.4ml/min 5cm x 2.0mm 2.4um 1.3ml/min 16 x Speed Enhancement

  8. Varian 920-LC Fast LC PAHs Varian 920-LC 6 minute Analysis Waters Acquity 7 Minute Analysis

  9. Varian 920-LC Solvent Delivery System • Dual piston quaternary pump produces fast gradients – even at 200 ml/min • Virtually pulse-free pumping without pulse damper • Outstanding gradient compositional accuracy give retention time precision at the <1% level • Built-in vacuum degas maintains pump prime and enhanced injection reliability • Accurate flow from 0.1 to 10 mL/min for 2 to 4.6 mm columns • Overlay of 10 chromatograms • 10% steps at 1ml/min

  10. Varian 920-LC Autosampler • Needle-in-flow path geometry for low carry-over (<0.01%) • Typical inj precision (< 0.3% at 10 ml) • Optimized flow paths for fast injection turn-around • Synchronised injection for best RT reproducibility • Programmable wash setting for low carry-over • Injection volumes from 1ul to 50mL • Cooled option available

  11. Varian 920-LC Washout Performance 125 ug/ml Caffeine Blank injection <0.01% carryover

  12. Varian 920-LC Injection Synchronization

  13. Sensitive UV-Vis Detectors Typical Agilent VWD S/N ~ 25 Typical Varian 920-LC UV/Vis S/N > 36

  14. Information Rich Diode Array Detection • 1024 pixels provides full coverage from 190 – 950 nm • Full l spectra collection • Enhanced purity • Automatic peak identification with customised libraries • Greater confidence on compound identification • Choice of flowcells • 4 mm path length semi-micro • 9mm path length analytical • Front panel access • Lamps and flowcells • D2 and Vis lamps self aligned • Easily user maintained

  15. Information Rich Diode Array Detection • Automatic peak identification with customised libraries • Use spectra as well as RT for compound ID • Greater confidence on compound identification • Peak search of unknown yields 998.7 match for naphthalene

  16. Varian 920-LC Fluorescence Detector • Sensitive Fluorescence detector (S/N > 600 Raman H2O peak baseline method) • Time programmable l for increase sensitivity • Automatic l validation with built-in Hg lamp • Low 12 ml dead volume flowcell for improved chromatography

  17. Varian 920-LC Refractive Index Detector • Based upon 356-LC RI detector • Low volume, flow efficient cell for superior peak shape • Stable, long life and high intensity LED • Minimises drift • Provides excellent detection limits • Enhanced life-time • Temperature controlled optics minimises baseline drift and provides outstanding detection limits • Complete automation thru Galaxie software

  18. Varian 380-LC and 385-LC ELSD • Universal HPLC detector • Compatible with gradients • More sensitive than RI • NEW Glass Nebuliser for Improved Reproducibility • 2 to 3 Times More Sensitive • 40Hz Data Rate for Fast LC • Complete Galaxie compatibility • Program changes thru a run

  19. Heated Column Compartment • Temperature range from ambient + 10oC up to 65oC • Excellent temperature precision +0.15oC • Ensures retention time reproducibility • Eliminates re-runs • Good temperature accuracy <1oC • Low delay volume pre-heater for efficient heat transfer to mobile phase • Rapid warm-up • Typically from ambient to 40oc in <15 minutes • Spacious - easily accommodates 2 x 250mm columns and 2 valves • Option with electrically actuated column switching valve and injection valve

  20. Excellent Long term Stability

  21. Quick Warmup - Outstanding Stability

  22. Reproducibility of 50 Injections Reproducibility <0.8% over 50 injections and 12 hours

  23. Varian 920-LC Software Usability • User friendly GalaxieTM software • Simplified, modern, consistent Instrument interface • Modular 11 pages • 920-LC 4 pages!! • One page real-time status and chromatogram • Advanced integration and calibration • New IMS capabilities • Automated instrument validation • Reduces downtime and operating costs • Fusion AE

  24. Varian 920-LC Integrated HPLC • Special features of the Varian 920-LC • Leak detector • Increases confidence with unattended automated runs • Single waste point • Ensures capture of all wastes • Easy to connect to waste vessel • Built-in Solvent bottle storage • Improved safety and convenience • Lower hight than modular systems

  25. Varian 920-LC Integrated HPLC • Special features of the Varian 920-LC • Clear front doors • Stylish • Provides access to autosampler without opening • Safety increased as lab staff shielded from solvent spray and leaks • Useful, convenient storage compartments • Column rack in door • Tool drawer • Operation log drawer

  26. Varian 920-LC AIQ (Validation) • AIQ (Analytical Instrument Qualification) • Fully PC controlled IQ and OQ (not PQ) • Puts the Varian 920-LC at the forefront of regulated capabilities • Consistent, reproducible methodology • Includes pumps, autosampler, detectors, column heater • Chargeable option • Fully documented

  27. 900 LC -Series AIQ • Full system test designed and implemented as part of the 900-LC Series Analytical Instrument Qualification package. • Verifies the entire LC system by systematically running the following • Flow Rate Accuracy Test • Wavelength Detector Accuracy Test • Auto-sampler Injector Accuracy Test (also measures Detector Linearity) • Auto-sampler Injector Precision Test • Auto-sampler Injector Carryover Test • Proportioning Accuracy Test • Detector Noise Test • Detector Drift Test • Auto-sampler Vial Identification Accuracy Test • Proportioning Linearity • Compositional Ripple

  28. Varian 920-LC Configurations

  29. Varian 920-LC Consumables • Excellent range of HPLC Columns providing industry leading resolution and robustness • Pursuit • Pursuit XRS • Polymeric Phases - PL • From 2mm to 2” ID Prepacked • Other Speciality Phases • NEW 2.8 Ultra Column AND 2.4um Columns for Fast or High Res LC • 2.8um Ultra is optimised for Speed • 2.4um gives further enhancements in Resolution

  30. 2.8um Vs 2.4 um – High Res or Extra Speed 2.4um 3mm x 5cm for RESOLUTION 2.8um 2mm x 5cm for speed

  31. Questions??

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